Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607013019_jr02 GTGCTTATGAGGTCATGGAAACCTGGTCTCAG(+ATATAT)GATCCATAAAAGATGATGTTTATAGTTAAGA NGG 10 8 17 8 24 0 100 5 10
    240607013018_8noj AAATATCTTAACTATAAACATCATCTTTTATG(+ATATAT)GATCCTGAGACCAGGTTTCCATGACCTCATA NGG 10 8 17 8 24 0 100 5 10
    240607013016_jo32 TGGTCTTTATTTAGAGTCCATAACCCCTCCAG(+ATATAT)GTACCTCAATCCTTCCCATAAATCTTCCACA NGG 10 8 17 8 24 0 100 5 10
    240607013015_794o GATCTTGTGGAAGATTTATGGGAAGGATTGAG(+ATATAT)GTACCTGGAGGGGTTATGGACTCTAAATAAA NGG 10 8 17 8 24 0 100 5 10
    240607013014_np3d GGTTAGACAGGCCAGCCCAGCTAGGAGAGCAG(+ATATAT)GATCCACAGGTAGACGGCAGAGTCATGGTAA NGG 10 8 17 8 24 0 100 5 10
    240607013012_9us9 GTGGCTTACCATGACTCTGCCGTCTACCTGTG(+ATATAT)GATCCTGCTCTCCTAGCTGGGCTGGCCTGTC NGG 10 8 17 8 24 0 100 5 10
    240607013010_h3kc GAAACAGCCAAAGAATGGATAAAGAAAATGAG(+ATATAT)GTACCTTTACATAATGGAATATTACTCAAAT NGG 10 8 17 8 24 0 100 5 10
    240607013009_jmox TTAATATTTGAGTAATATTCCATTATGTAAAG(+ATATAT)GTACCTCATTTTCTTTATCCATTCTTTGGCT NGG 10 8 17 8 24 0 100 5 10
    240607013008_ljwc ATAATAGGGTAATGCTTAGTAGAAGGCACGTG(+ATATAT)GATCCATACACTAGACTAACTCGGGAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240607013007_2juz AATTTTCCACTCCCGAGTTAGTCTAGTGTATG(+ATATAT)GATCCACGTGCCTTCTACTAAGCATTACCCT NGG 10 8 17 8 24 0 100 5 10