Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607013047_55na AGTAGATGGAGATAAAAGAAATGAATTCCCAG(+ATATAT)GTACCAAATTTATTCCGAAGGTTTAGAAACG NGG 10 8 17 8 24 0 100 5 10
    240607013046_3tbq TTCAGCGTTTCTAAACCTTCGGAATAAATTTG(+ATATAT)GTACCTGGGAATTCATTTCTTTTATCTCCAT NGG 10 8 17 8 24 0 100 5 10
    240607013045_kogl ATGATAAGATAATGACTAATTTATAGTTCAAG(+ATATAT)GATCCTTCTAATGAGGAAGCCAATGGTTAGA NGG 10 8 17 8 24 0 100 5 10
    240607013044_inmj ACCACTCTAACCATTGGCTTCCTCATTAGAAG(+ATATAT)GATCCTTGAACTATAAATTAGTCATTATCTT NGG 10 8 17 8 24 0 100 5 10
    240607013043_6e5d TTGGAAGCAAGAAAAACAGACACGCAGGCAAG(+ATATAT)GTACCTGATCTCACAGGCCTAAGGCCTAAGA NGG 10 8 17 8 24 0 100 5 10
    240607013042_upuo GTGCTTCTTAGGCCTTAGGCCTGTGAGATCAG(+ATATAT)GTACCTTGCCTGCGTGTCTGTTTTTCTTGCT NGG 10 8 17 8 24 0 100 5 10
    240607013040_q6yr TATTTGGCTGTAGCTCTGATCAACTTTCCCTG(+ATATAT)GTACCCTCATCCTACCTTCATATACAGGTAG NGG 10 8 17 8 24 0 100 5 10
    240607013038_kj9y TGGAGCTACCTGTATATGAAGGTAGGATGAGG(+ATATAT)GTACCAGGGAAAGTTGATCAGAGCTACAGCC NGG 10 8 17 8 24 0 100 5 10
    240607013036_xppp CATCCCAAAGTTGGCCTTTGTCCTTCCCCATG(+ATATAT)GATCCCTAAAAACAAACAAACAAATAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240607013034_keiu TTTATTTTTTTATTTGTTTGTTTGTTTTTAGG(+ATATAT)GATCCATGGGGAAGGACAAAGGCCAACTTTG NGG 10 8 17 8 24 0 100 5 10