Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607013131_ehzm ACCTGCATCTTTGTTCCCTAAATTAGTGCTTG(+ATATAT)GTACCCAGGAAGATCAATTAGTATGTACTGA NGG 10 8 17 8 24 0 100 5 10
    240607013129_l9se TTAACTCAGTACATACTAATTGATCTTCCTGG(+ATATAT)GTACCAAGCACTAATTTAGGGAACAAAGATG NGG 10 8 17 8 24 0 100 5 10
    240607013128_ao0k AACTGTACAATCCCAAGATAACTTGAATATAG(+ATATAT)GTACCAAAGTTACATAAAAATTCAGTCAAAA NGG 10 8 17 8 24 0 100 5 10
    240607013127_v0nj TTACATTTTGACTGAATTTTTATGTAACTTTG(+ATATAT)GTACCTATATTCAAGTTATCTTGGGATTGTA NGG 10 8 17 8 24 0 100 5 10
    240607013125_ohin GGGAATCCAGCACCCTTTTCTGGACTCCATGG(+ATATAT)GTACCTGCAATCACACATGATGTGCATGCAC NGG 10 8 17 8 24 0 100 5 10
    240607013124_t0gx TGTCTGTGCATGCACATCATGTGTGATTGCAG(+ATATAT)GTACCCATGGAGTCCAGAAAAGGGTGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240607013123_hhf7 TATTTGTGTGTGGAGAGAGAAAGAGACTGAAG(+ATATAT)GATCCCCTTCCCTTTTTTTCTTTGTGCTAGG NGG 10 8 17 8 24 0 100 5 10
    240607013121_y6ra GAGGTCCTAGCACAAAGAAAAAAAGGGAAGGG(+ATATAT)GATCCTTCAGTCTCTTTCTCTCTCCACACAC NGG 10 8 17 8 24 0 100 5 10
    240607013120_ff04 AAGTAGGGTGAGGCCTTAAGCTTTCAAATCTG(+ATATAT)GATCCCAGGGAAGCACTTCCTTTAACAAGGC NGG 10 8 17 8 24 0 100 5 10
    240607013117_r1v9 TGGTGGCCTTGTTAAAGGAAGTGCTTCCCTGG(+ATATAT)GATCCAGATTTGAAAGCTTAAGGCCTCACCC NGG 10 8 17 8 24 0 100 5 10