Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607013217_dbll AAGGAACATTTAACATCAAGATTTAAACAGTG(+ATATAT)GTACCACTTGGGTGGCAAACTGGTTCTTAAA NGG 10 8 17 8 24 0 100 5 10
    240607013216_unao AGATCTTTAAGAACCAGTTTGCCACCCAAGTG(+ATATAT)GTACCACTGTTTAAATCTTGATGTTAAATGT NGG 10 8 17 8 24 0 100 5 10
    240607013214_mk2r AAACATTCCATGTTCCATTGTCGCTCCTCCGG(+ATATAT)GTACCCAGTTGGGACCCTGGTGCCAGCATTT NGG 10 8 17 8 24 0 100 5 10
    240607013212_p9or CACTGAAATGCTGGCACCAGGGTCCCAACTGG(+ATATAT)GTACCCGGAGGAGCGACAATGGAACATGGAA NGG 10 8 17 8 24 0 100 5 10
    240607013211_3e0m CAGTCATGCCCAGCTTTCATAGGGGTTCTGGG(+ATATAT)GATCCAAACTTAGGTCTTCACACATGCTCAC NGG 10 8 17 8 24 0 100 5 10
    240607013209_7cpu ACGTGGTGAGCATGTGTGAAGACCTAAGTTTG(+ATATAT)GATCCCCAGAACCCCTATGAAAGCTGGGCAT NGG 10 8 17 8 24 0 100 5 10
    240607013208_kdgd TGGACAGCCATGTTGCTATTTGGCATGAAGAG(+ATATAT)GATCCCACTCAAAGATCCAAGGGAGGAATCT NGG 10 8 17 8 24 0 100 5 10
    240607013207_u3p0 TCCCAAGATTCCTCCCTTGGATCTTTGAGTGG(+ATATAT)GATCCTCTTCATGCCAAATAGCAACATGGCT NGG 10 8 17 8 24 0 100 5 10
    240607013205_j7vg CAGATAGCCTTTGATGCATATAAAGGACAGCG(+ATATAT)GATCCGCTGCACTCCAGGTGCTGCTCAAGCC NGG 10 8 17 8 24 0 100 5 10
    240607013204_s6h2 TAGGAGGCTTGAGCAGCACCTGGAGTGCAGCG(+ATATAT)GATCCGCTGTCCTTTATATGCATCAAAGGCT NGG 10 8 17 8 24 0 100 5 10