Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607013148_ez0n TCATATAGAATCTAGGCGTGGCCTCCCTCCTG(+ATATAT)GTACCATTCTGGTATCCAACACCAATTACTT NGG 10 8 17 8 24 0 100 5 10
    240607013147_rv30 AGGTGAAGTAATTGGTGTTGGATACCAGAATG(+ATATAT)GTACCAGGAGGGAGGCCACGCCTAGATTCTA NGG 10 8 17 8 24 0 100 5 10
    240607013145_0j6y CCAAAGGACTTTGCTGACCACTACTGCATGGG(+ATATAT)GATCCAACCAATTCAATTAATGTCTTCATAT NGG 10 8 17 8 24 0 100 5 10
    240607013144_p7kb CTTCAATATGAAGACATTAATTGAATTGGTTG(+ATATAT)GATCCCCATGCAGTAGTGGTCAGCAAAGTCC NGG 10 8 17 8 24 0 100 5 10
    240607013142_szuu AGTTATCACGATACAGTAGAGAACTGATATGG(+ATATAT)GATCCTGAGGATGTCTTCAAAGGGACCTCTA NGG 10 8 17 8 24 0 100 5 10
    240607013140_hw19 AAGCCTAGAGGTCCCTTTGAAGACATCCTCAG(+ATATAT)GATCCCATATCAGTTCTCTACTGTATCGTGA NGG 10 8 17 8 24 0 100 5 10
    240607013139_e27s AGTTATCACGATACAGTAGAGAACTGATATGG(+ATATAT)GATCCTGAGGATGTCTTCAAAGGGACCTCTA NGG 10 8 17 8 24 0 100 5 10
    240607013137_357j AAGCCTAGAGGTCCCTTTGAAGACATCCTCAG(+ATATAT)GATCCCATATCAGTTCTCTACTGTATCGTGA NGG 10 8 17 8 24 0 100 5 10
    240607013135_kz3l GAGTTCACAGCTGAGGTCTCATGCGACCCCAG(+ATATAT)GATCCCCCAGCCAATGATAAAGCACAGTGCA NGG 10 8 17 8 24 0 100 5 10
    240607013132_zpu2 GTTACTGCACTGTGCTTTATCATTGGCTGGGG(+ATATAT)GATCCTGGGGTCGCATGAGACCTCAGCTGTG NGG 10 8 17 8 24 0 100 5 10