Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607013103_plxd AATTTTCATTTGTGTAAACTCCAAAAAAAAAG(+ATATAT)GATCCTAACAACTATTTTGGGTTTTATCATT NGG 10 8 17 8 24 0 100 5 10
    240607013102_65h2 AAGTAAATGATAAAACCCAAAATAGTTGTTAG(+ATATAT)GATCCTTTTTTTTTGGAGTTTACACAAATGA NGG 10 8 17 8 24 0 100 5 10
    240607013100_b5b0 TAATATTTAATGATTGAAATTAACTGTTTGGG(+ATATAT)GATCCTGATGCTGATGTGTGTATTACTAAAG NGG 10 8 17 8 24 0 100 5 10
    240607013059_5oi3 AAATTCTTTAGTAATACACACATCAGCATCAG(+ATATAT)GATCCCCAAACAGTTAATTTCAATCATTAAA NGG 10 8 17 8 24 0 100 5 10
    240607013058_yy01 TTTGATGCCAACAAAGTTTTAGGGATACAATG(+ATATAT)GTACCTTGCCAACATCGAGTGACTTTAGCTG NGG 10 8 17 8 24 0 100 5 10
    240607013056_2z80 TCTTCCAGCTAAAGTCACTCGATGTTGGCAAG(+ATATAT)GTACCATTGTATCCCTAAAACTTTGTTGGCA NGG 10 8 17 8 24 0 100 5 10
    240607013055_g16c AAGCTGACGCTACTGACGGGCTTGGGGGAAGG(+ATATAT)GATCCCCTGGGTTCCTGGGTTCCTCAGATAT NGG 10 8 17 8 24 0 100 5 10
    240607013052_4gix GCCAAATATCTGAGGAACCCAGGAACCCAGGG(+ATATAT)GATCCCTTCCCCCAAGCCCGTCAGTAGCGTC NGG 10 8 17 8 24 0 100 5 10
    240607013051_mvw1 CTCGAAAAAGCACAATCGAAGCTTCCCAAAGG(+ATATAT)GATCCAGACTCCTTCGAGCTGATCCGAGCCT NGG 10 8 17 8 24 0 100 5 10
    240607013050_22dj AAAGAAGGCTCGGATCAGCTCGAAGGAGTCTG(+ATATAT)GATCCCTTTGGGAAGCTTCGATTGTGCTTTT NGG 10 8 17 8 24 0 100 5 10