Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607013033_pxcr AGGTTCCAGACTTCTCCCAGCCACCGTGGCAG(+ATATAT)GTACCCCTCATGTCAGGGACTGCCTCGTGCT NGG 10 8 17 8 24 0 100 5 10
    240607013031_4xtq AAAGCAGCACGAGGCAGTCCCTGACATGAGGG(+ATATAT)GTACCTGCCACGGTGGCTGGGAGAAGTCTGG NGG 10 8 17 8 24 0 100 5 10
    240607013030_ar5p TAGTTAGCGATGAGGAGGAAGACACTTGAATG(+ATATAT)GATCCCCACCCTAGGATGCTGCTCTTCTTGC NGG 10 8 17 8 24 0 100 5 10
    240607013028_jolk GGAAGGCAAGAAGAGCAGCATCCTAGGGTGGG(+ATATAT)GATCCATTCAAGTGTCTTCCTCCTCATCGCT NGG 10 8 17 8 24 0 100 5 10
    240607013027_b8w9 TATTGATTCTCTTAGAACTTTGTCAGTGGATG(+ATATAT)GTACCTTGCCTTTGCAGGTTGTATGTGACCT NGG 10 8 17 8 24 0 100 5 10
    240607013026_zzyf ATCTAAGGTCACATACAACCTGCAAAGGCAAG(+ATATAT)GTACCATCCACTGACAAAGTTCTAAGAGAAT NGG 10 8 17 8 24 0 100 5 10
    240607013024_hd2q CAAACAGAGACAAGCTTCTCTGAATGTGGGTG(+ATATAT)GTACCATTCCCATAGGCTTTGGCCAAGGAGA NGG 10 8 17 8 24 0 100 5 10
    240607013023_wnml TCTACTCTCCTTGGCCAAAGCCTATGGGAATG(+ATATAT)GTACCACCCACATTCAGAGAAGCTTGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240607013022_peb1 GCTTGGAAGCTTGAGACCCAAATAGAAGTTTG(+ATATAT)GATCCAATGACTGCAAACTGCTCCTTTTCAG NGG 10 8 17 8 24 0 100 5 10
    240607013021_wdzk TCTCTCTGAAAAGGAGCAGTTTGCAGTCATTG(+ATATAT)GATCCAAACTTCTATTTGGGTCTCAAGCTTC NGG 10 8 17 8 24 0 100 5 10