Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607012951_af1h AAACAAACCAAACAAAACATAAAAAGCCTTTG(+ATATAT)GTACCTTTCAATAACAAAAGAAAAATATATT NGG 10 8 17 8 24 0 100 5 10
    240607012950_yadq ATCTAAATATATTTTTCTTTTGTTATTGAAAG(+ATATAT)GTACCAAAGGCTTTTTATGTTTTGTTTGGTT NGG 10 8 17 8 24 0 100 5 10
    240607012949_2dy0 GAGGCAACTGCAGACGAATCAGAAAATAAGAG(+ATATAT)GATCCACCACCACAGAACTGATTGGCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240607012947_2ets ATTGCTCTGAGCCAATCAGTTCTGTGGTGGTG(+ATATAT)GATCCTCTTATTTTCTGATTCGTCTGCAGTT NGG 10 8 17 8 24 0 100 5 10
    240607012946_xq8d GTAAGAATAAGTTGATAGCTTCAAATCTACAG(+ATATAT)GATCCCTTCAGCTGGCACTATCTCCCTAAAA NGG 10 8 17 8 24 0 100 5 10
    240607012945_1qif AGACATTTTAGGGAGATAGTGCCAGCTGAAGG(+ATATAT)GATCCTGTAGATTTGAAGCTATCAACTTATT NGG 10 8 17 8 24 0 100 5 10
    240607012943_1r8u TTACAGGGAAATTCATTATAGATATTCTTTTG(+ATATAT)GTACCTGTGAATTAAATATAAATATAAGATG NGG 10 8 17 8 24 0 100 5 10
    240607012942_23v0 GAGTTCATCTTATATTTATATTTAATTCACAG(+ATATAT)GTACCAAAAGAATATCTATAATGAATTTCCC NGG 10 8 17 8 24 0 100 5 10
    240607012941_vc9d ATACCCCTTGGCTATGCCCACAAATCCATAAG(+ATATAT)GATCCTGACTTAGAAAGAAAAACAGCACAAA NGG 10 8 17 8 24 0 100 5 10
    240607012940_tse0 TTGAATTTGTGCTGTTTTTCTTTCTAAGTCAG(+ATATAT)GATCCTTATGGATTTGTGGGCATAGCCAAGG NGG 10 8 17 8 24 0 100 5 10