Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607013232_8ydp CCCATTTCCGTTCACAGTATGTTGGGGCCCAG(+ATATAT)GTACCAACAGTGTAGGGCTTTCTGATACCCT NGG 10 8 17 8 24 0 100 5 10
    240607013231_c8p9 TCCTGAGGGTATCAGAAAGCCCTACACTGTTG(+ATATAT)GTACCTGGGCCCCAACATACTGTGAACGGAA NGG 10 8 17 8 24 0 100 5 10
    240607013230_xki8 GGGGAGGGAGATGAGGCTGCCCCAAGGCTCTG(+ATATAT)GTACCTGGCTTTAGTTTTCTGTCTTCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240607013228_2kxv CCTGAGGAAAGAAGACAGAAAACTAAAGCCAG(+ATATAT)GTACCAGAGCCTTGGGGCAGCCTCATCTCCC NGG 10 8 17 8 24 0 100 5 10
    240607013227_ts0a GAGTATTATGGAGCCCGTGGCTTTGTGCTATG(+ATATAT)GATCCTGGGATGGGGGCCTCTTACCCCCCAC NGG 10 8 17 8 24 0 100 5 10
    240607013225_4gup GGCTAGTGGGGGGTAAGAGGCCCCCATCCCAG(+ATATAT)GATCCATAGCACAAAGCCACGGGCTCCATAA NGG 10 8 17 8 24 0 100 5 10
    240607013223_108r CAGACTGGGTAAGATGTGGCTCTCTCAGGTGG(+ATATAT)GATCCCTTTATTTATTTACTCAAGTCCCCAT NGG 10 8 17 8 24 0 100 5 10
    240607013221_mqsa TTTGTATGGGGACTTGAGTAAATAAATAAAGG(+ATATAT)GATCCCACCTGAGAGAGCCACATCTTACCCA NGG 10 8 17 8 24 0 100 5 10
    240607013220_ej5k TTATTATTCATTTAACCATCCATCCACACAAG(+ATATAT)GTACCTTCTTGAAACCTCTGGGCAGTCTCAC NGG 10 8 17 8 24 0 100 5 10
    240607013219_8q42 CAAATGTGAGACTGCCCAGAGGTTTCAAGAAG(+ATATAT)GTACCTTGTGTGGATGGATGGTTAAATGAAT NGG 10 8 17 8 24 0 100 5 10