Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607013333_r0d7 GTAGCTCTCAAGACGTTCCCTGCACCTGACTG(+ATATAT)GTACCTTCCTGAGCCTCAGCTTTATCTTTAC NGG 10 8 17 8 24 0 100 5 10
    240607013332_j23u ACTAGGTAAAGATAAAGCTGAGGCTCAGGAAG(+ATATAT)GTACCAGTCAGGTGCAGGGAACGTCTTGAGA NGG 10 8 17 8 24 0 100 5 10
    240607013330_2ga1 CCTCATGGGATATCCTAAAGAAGGCAGCCTGG(+ATATAT)GATCCTACCAGAAACTGATTGCAGCAGACCT NGG 10 8 17 8 24 0 100 5 10
    240607013329_kwp7 TGTCCAGGTCTGCTGCAATCAGTTTCTGGTAG(+ATATAT)GATCCCAGGCTGCCTTCTTTAGGATATCCCA NGG 10 8 17 8 24 0 100 5 10
    240607013328_tek0 AAGGAAAGGCAGCAGTATGCAAAGTGGAGAAG(+ATATAT)GTACCTAAGTCCATACTGCCCACAGTAATAG NGG 10 8 17 8 24 0 100 5 10
    240607013326_qrnb AAGAACTATTACTGTGGGCAGTATGGACTTAG(+ATATAT)GTACCTTCTCCACTTTGCATACTGCTGCCTT NGG 10 8 17 8 24 0 100 5 10
    240607013325_tady CTGTGAATTATGCATAGCCCCTGCCACTTCAG(+ATATAT)GATCCTCTTTGCCCAAGTGTACTGAGGACCA NGG 10 8 17 8 24 0 100 5 10
    240607013324_rira AACAATGGTCCTCAGTACACTTGGGCAAAGAG(+ATATAT)GATCCTGAAGTGGCAGGGGCTATGCATAATT NGG 10 8 17 8 24 0 100 5 10
    240607013322_71ei CATGCCTTTTCTTGATGTCTGAGTTATTTCGG(+ATATAT)GTACCTTTTAGGGAGGAATGCCTTTTGCAAT NGG 10 8 17 8 24 0 100 5 10
    240607013321_ef7p ACATTATTGCAAAAGGCATTCCTCCCTAAAAG(+ATATAT)GTACCCGAAATAACTCAGACATCAAGAAAAG NGG 10 8 17 8 24 0 100 5 10