Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607013424_ufua GCTGGCAGGCCCTGAAGCACAAGTTCAGGCCG(+ATATAT)GATCCGGGCATCAGGACTTCGTGTTCATACA NGG 10 8 17 8 24 0 100 5 10
    240607013422_bm5m GTCCATGTATGAACACGAAGTCCTGATGCCCG(+ATATAT)GATCCGGCCTGAACTTGTGCTTCAGGGCCTG NGG 10 8 17 8 24 0 100 5 10
    240607013420_k8k3 CATGACAACAGTGGTGTCCACTCCCACATTCG(+ATATAT)GATCCCAGGGTCCTACGTCCTCCTAAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240607013418_6rtg CCCGACTGCTTTAGGAGGACGTAGGACCCTGG(+ATATAT)GATCCGAATGTGGGAGTGGACACCACTGTTG NGG 10 8 17 8 24 0 100 5 10
    240607013416_yvg7 ATCGGCTGATGAGACCGGGTTCCATCTAAGCG(+ATATAT)GTACCTGACTAACTCTGGCTCACTAGGGCTG NGG 10 8 17 8 24 0 100 5 10
    240607013415_1ewk GGGAACAGCCCTAGTGAGCCAGAGTTAGTCAG(+ATATAT)GTACCGCTTAGATGGAACCCGGTCTCATCAG NGG 10 8 17 8 24 0 100 5 10
    240607013413_kn7w ACTAACTTGAGGTGAGGCAGCAAAAGGGCCTG(+ATATAT)GTACCACCAAATGGTGGCTTCCACCCCGTGG NGG 10 8 17 8 24 0 100 5 10
    240607013412_f771 TGACCCCACGGGGTGGAAGCCACCATTTGGTG(+ATATAT)GTACCAGGCCCTTTTGCTGCCTCACCTCAAG NGG 10 8 17 8 24 0 100 5 10
    240607013410_4mrt AGACCAGCCTTCTCAGGTTCTCTGGCTCGTGG(+ATATAT)GATCCTTCCAGGGAGCTACCTACAGAGCTTG NGG 10 8 17 8 24 0 100 5 10
    240607013409_49hu TAACCCAAGCTCTGTAGGTAGCTCCCTGGAAG(+ATATAT)GATCCCACGAGCCAGAGAACCTGAGAAGGCT NGG 10 8 17 8 24 0 100 5 10