Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607013506_2qkw CCAGTCTGGAAATGAAAGCTGCTTCCAGTCAG(+ATATAT)GATCCATTGTAAGAAAAGTGCGTTCGTGAGC NGG 10 8 17 8 24 0 100 5 10
    240607013505_wb3f TCCATGCTCACGAACGCACTTTTCTTACAATG(+ATATAT)GATCCTGACTGGAAGCAGCTTTCATTTCCAG NGG 10 8 17 8 24 0 100 5 10
    240607013503_krng GCCCACTGGAGGAATCCGGGGCTTGGCAGTGG(+ATATAT)GATCCTGCTGAACTGTACTTCGAATACATCC NGG 10 8 17 8 24 0 100 5 10
    240607013502_3uog AATTTGGATGTATTCGAAGTACAGTTCAGCAG(+ATATAT)GATCCCACTGCCAAGCCCCGGATTCCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240607013501_zc3z TCCCTCCGTTCTGGGCTGTTAGCACCACTCAG(+ATATAT)GTACCAAGGACTGCTCTGAGCACAGTAGAGA NGG 10 8 17 8 24 0 100 5 10
    240607013459_1yz0 AAGTCTCTCTACTGTGCTCAGAGCAGTCCTTG(+ATATAT)GTACCTGAGTGGTGCTAACAGCCCAGAACGG NGG 10 8 17 8 24 0 100 5 10
    240607013458_7sfm AGCCAACAGGAAAAACTACTGAAAATGTCATG(+ATATAT)GATCCCATGTCAGTCTGGCACTTGATTCTTT NGG 10 8 17 8 24 0 100 5 10
    240607013456_s4up GGCACAAAGAATCAAGTGCCAGACTGACATGG(+ATATAT)GATCCATGACATTTTCAGTAGTTTTTCCTGT NGG 10 8 17 8 24 0 100 5 10
    240607013455_u9o3 CACTAACCACAACACAGTACAAGTGTCTCTGG(+ATATAT)GTACCCCGAACATCTCCCCGAAGAGTTCTAG NGG 10 8 17 8 24 0 100 5 10
    240607013453_m50t GAGGACTAGAACTCTTCGGGGAGATGTTCGGG(+ATATAT)GTACCCAGAGACACTTGTACTGTGTTGTGGT NGG 10 8 17 8 24 0 100 5 10