Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607013452_3z25 ATATTCATGTTTATTCGCTCTCTACCAAGTAG(+ATATAT)GATCCATTTTTGATAAAGCAGTCTTTGGGTT NGG 10 8 17 8 24 0 100 5 10
    240607013450_gp22 GAAGAAACCCAAAGACTGCTTTATCAAAAATG(+ATATAT)GATCCTACTTGGTAGAGAGCGAATAAACATG NGG 10 8 17 8 24 0 100 5 10
    240607013449_vvx5 GTGTGGTGCAACCTAGAGCCCATCAGAGGTGG(+ATATAT)GTACCTATGTATACAAGTCCCACCTGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240607013448_zg7l CTGACAAAGCCAGGTGGGACTTGTATACATAG(+ATATAT)GTACCCACCTCTGATGGGCTCTAGGTTGCAC NGG 10 8 17 8 24 0 100 5 10
    240607013446_bkma ACTGGAAGCCCAAGCCAAACGCCCAAGCCTCG(+ATATAT)GTACCTATTAGGACTTACACTGACCTCGCAC NGG 10 8 17 8 24 0 100 5 10
    240607013445_46rd TCCGTGTGCGAGGTCAGTGTAAGTCCTAATAG(+ATATAT)GTACCGAGGCTTGGGCGTTTGGCTTGGGCTT NGG 10 8 17 8 24 0 100 5 10
    240607013443_9g92 TTTAAAAATATATATATAAACTAAATCACACG(+ATATAT)GTACCAAAGCTGTAATGGAAAAAGGAAAGAG NGG 10 8 17 8 24 0 100 5 10
    240607013442_p87m CTTAACTCTTTCCTTTTTCCATTACAGCTTTG(+ATATAT)GTACCGTGTGATTTAGTTTATATATATATTT NGG 10 8 17 8 24 0 100 5 10
    240607013441_f3cz CAGCACATCCTAGGCAGGGCCGAACCAGCATG(+ATATAT)GTACCAAGTGCAAAGATCCGACTTACCTACA NGG 10 8 17 8 24 0 100 5 10
    240607013440_wqtb CGATCTGTAGGTAAGTCGGATCTTTGCACTTG(+ATATAT)GTACCATGCTGGTTCGGCCCTGCCTAGGATG NGG 10 8 17 8 24 0 100 5 10