Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607013438_2fr3 CCTCGTGTCCACCGCAGGCTTTTGGTACAAGG(+ATATAT)GTACCTCATTTGCGTTTTTTAAAACCAGTCT NGG 10 8 17 8 24 0 100 5 10
    240607013437_2dxt AAGGCAGACTGGTTTTAAAAAACGCAAATGAG(+ATATAT)GTACCCTTGTACCAAAAGCCTGCGGTGGACA NGG 10 8 17 8 24 0 100 5 10
    240607013436_rnnl GAACACAAGGAAACTCAGTCATGCTCAGAAAG(+ATATAT)GATCCAGCTAAGTGAGCAGGTGGACTCCAAG NGG 10 8 17 8 24 0 100 5 10
    240607013435_ub30 TGAGCCTTGGAGTCCACCTGCTCACTTAGCTG(+ATATAT)GATCCTTTCTGAGCATGACTGAGTTTCCTTG NGG 10 8 17 8 24 0 100 5 10
    240607013433_kv0w GCTGTTTTACAGCAGTGTGCCAAGAGATGTGG(+ATATAT)GATCCACAGAGGGATTTTCTAACCTGGAGCA NGG 10 8 17 8 24 0 100 5 10
    240607013432_j2jc GCCCCTGCTCCAGGTTAGAAAATCCCTCTGTG(+ATATAT)GATCCCACATCTCTTGGCACACTGCTGTAAA NGG 10 8 17 8 24 0 100 5 10
    240607013431_hqco GAAAGGACAGGGTGTTCAGCACGTGTAGTTGG(+ATATAT)GTACCTGGCCTAGAGGATACCCCTGAAGCTT NGG 10 8 17 8 24 0 100 5 10
    240607013429_nguh CTAAGAAGCTTCAGGGGTATCCTCTAGGCCAG(+ATATAT)GTACCCAACTACACGTGCTGAACACCCTGTC NGG 10 8 17 8 24 0 100 5 10
    240607013427_o61x GTAGGCCTGGCTAGCTAGCCAGTTTGCACTGG(+ATATAT)GTACCCAGACTGGAATGGCAGGTGAGCCACC NGG 10 8 17 8 24 0 100 5 10
    240607013426_9t2z AACCAGGTGGCTCACCTGCCATTCCAGTCTGG(+ATATAT)GTACCCAGTGCAAACTGGCTAGCTAGCCAGG NGG 10 8 17 8 24 0 100 5 10