Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607013407_4uch CCAAGGAATGGAAGGCCCAGGTCTTTCTCCAG(+ATATAT)GTACCGTACAGCTGGCCCCTTTGCTTACTGC NGG 10 8 17 8 24 0 100 5 10
    240607013406_asei GCAGAGCAGTAAGCAAAGGGGCCAGCTGTACG(+ATATAT)GTACCTGGAGAAAGACCTGGGCCTTCCATTC NGG 10 8 17 8 24 0 100 5 10
    240607013405_uhkx TCAATGGTGCTTTAAGGACGTGTGCAGGATAG(+ATATAT)GTACCTTAGAGGAGCAGCCTAGTTTCTCTGC NGG 10 8 17 8 24 0 100 5 10
    240607013403_x04d CATCAGCAGAGAAACTAGGCTGCTCCTCTAAG(+ATATAT)GTACCTATCCTGCACACGTCCTTAAAGCACC NGG 10 8 17 8 24 0 100 5 10
    240607013402_uxf4 GTCCTGTTAGATGGAATCCTGTCATCTCATTG(+ATATAT)GTACCCAGGGAGCCCAACTTCTTAGTAGCTC NGG 10 8 17 8 24 0 100 5 10
    240607013359_e8ry CAAGAGAGCTACTAAGAAGTTGGGCTCCCTGG(+ATATAT)GTACCAATGAGATGACAGGATTCCATCTAAC NGG 10 8 17 8 24 0 100 5 10
    240607013358_mt4w TGGGTAACTACGGAGGCCAAAAGAAGGCATTG(+ATATAT)GATCCCCTGGAGCTGTAGTTACAGGTGCTTG NGG 10 8 17 8 24 0 100 5 10
    240607013356_jmyq GCTCACAAGCACCTGTAACTACAGCTCCAGGG(+ATATAT)GATCCAATGCCTTCTTTTGGCCTCCGTAGTT NGG 10 8 17 8 24 0 100 5 10
    240607013354_h95g GGCAGGTGAGTAGCTCTGAGCTCCTGCCTCAG(+ATATAT)GTACCTTAACATGGACAGCAATGTGGAAACT NGG 10 8 17 8 24 0 100 5 10
    240607013353_7x6m ATTCCAGTTTCCACATTGCTGTCCATGTTAAG(+ATATAT)GTACCTGAGGCAGGAGCTCAGAGCTACTCAC NGG 10 8 17 8 24 0 100 5 10