Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607013320_34kq GCCCAGTGAGAGAACAGGAAGGTACAGGATAG(+ATATAT)GTACCCCCTCTTCCTAGATTGTAGTGGCCAG NGG 10 8 17 8 24 0 100 5 10
    240607013317_b3ma CTCCCCTGGCCACTACAATCTAGGAAGAGGGG(+ATATAT)GTACCTATCCTGTACCTTCCTGTTCTCTCAC NGG 10 8 17 8 24 0 100 5 10
    240607013316_weum TCTGCTCGTGGTAGAGAGGAAGCTGTGTGTGG(+ATATAT)GTACCAAGGGCTGCCAGTCTTTTCAGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240607013313_2fhj CTCCGTGCCTCTGAAAAGACTGGCAGCCCTTG(+ATATAT)GTACCCACACACAGCTTCCTCTCTACCACGA NGG 10 8 17 8 24 0 100 5 10
    240607013312_x0kq GATCTATCTGGAGTGGAGCCAGGGGTCATAAG(+ATATAT)GTACCCAGAAGGAGCTGGCTATGGCTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240607013311_z88o TGATATTTTCAGCCATAGCCAGCTCCTTCTGG(+ATATAT)GTACCTTATGACCCCTGGCTCCACTCCAGAT NGG 10 8 17 8 24 0 100 5 10
    240607013309_2qgx CTCTGGGGTCAAATCAATTCCTCTCAAGTGTG(+ATATAT)GTACCCTAGAGAGTGGTGGCCATCTACATTT NGG 10 8 17 8 24 0 100 5 10
    240607013308_4pp1 GGAGAAAATGTAGATGGCCACCACTCTCTAGG(+ATATAT)GTACCACACTTGAGAGGAATTGATTTGACCC NGG 10 8 17 8 24 0 100 5 10
    240607013306_fka9 GGCTCCGATTCCTTCACATTCCAGCTGGCCCG(+ATATAT)GATCCCTGTGTGGCCATAAGGCCTTGCCCTC NGG 10 8 17 8 24 0 100 5 10
    240607013304_sqib GTGACGAGGGCAAGGCCTTATGGCCACACAGG(+ATATAT)GATCCGGGCCAGCTGGAATGTGAAGGAATCG NGG 10 8 17 8 24 0 100 5 10