Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607013352_6hnj AGGTACTTCCTAAAGCTGCCCTGCCTTTACAG(+ATATAT)GATCCCCCGAAAGAGAATTTAGGACGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240607013349_1ihi CAGGCTGCCTCGTCCTAAATTCTCTTTCGGGG(+ATATAT)GATCCTGTAAAGGCAGGGCAGCTTTAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240607013348_s2rq ATGGGGCTGCAGGACGGATGGCCTGTCTCTAG(+ATATAT)GATCCCACTTTCAAGGCAACAGTCTGGCCAA NGG 10 8 17 8 24 0 100 5 10
    240607013347_7zjc AGGCCTTGGCCAGACTGTTGCCTTGAAAGTGG(+ATATAT)GATCCTAGAGACAGGCCATCCGTCCTGCAGC NGG 10 8 17 8 24 0 100 5 10
    240607013345_uu4d TACATTTTCACCTCCAGTCAGCAATTTCCTAG(+ATATAT)GTACCATGTAAAGCTGTAAAATTGAATTATT NGG 10 8 17 8 24 0 100 5 10
    240607013344_wkj5 TCAAGAATAATTCAATTTTACAGCTTTACATG(+ATATAT)GTACCTAGGAAATTGCTGACTGGAGGTGAAA NGG 10 8 17 8 24 0 100 5 10
    240607013341_tw67 AGGATGTTGGTGACCAGTCCCGAGGTCCCCAG(+ATATAT)GATCCGGGAAGCAGCAGGGTCCTGAGCACTA NGG 10 8 17 8 24 0 100 5 10
    240607013339_mrtd TTGTGTAGTGCTCAGGACCCTGCTGCTTCCCG(+ATATAT)GATCCTGGGGACCTCGGGACTGGTCACCAAC NGG 10 8 17 8 24 0 100 5 10
    240607013337_q1rn AGAGAATGTGGGGACTGCCAGGGGGGCCAGTG(+ATATAT)GATCCCTGGCCTTCTTGTCTGTACTAAGGCT NGG 10 8 17 8 24 0 100 5 10
    240607013335_aiun ACACAAGCCTTAGTACAGACAAGAAGGCCAGG(+ATATAT)GATCCACTGGCCCCCCTGGCAGTCCCCACAT NGG 10 8 17 8 24 0 100 5 10