Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607012834_5squ AAAAAGAGTTTGAACTGTATTGTAAAGAGCTG(+ATATAT)GTACCTGAAGCAACCATGTATTTTGCAAGAT NGG 10 8 17 8 24 0 100 5 10
    240607012833_4a23 CCAAGCTTTTTTTTTTTTAAGGCAAATTTTTG(+ATATAT)GTACCTTTTTTCCTAAACATCTTCGTGAATA NGG 10 8 17 8 24 0 100 5 10
    240607012832_owt0 TTTAATATTCACGAAGATGTTTAGGAAAAAAG(+ATATAT)GTACCAAAAATTTGCCTTAAAAAAAAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240607012830_p9ak ATGAACACACACATAATCCAGCACGTAGTAGG(+ATATAT)GATCCTCCTCGCCTAGGCTAAATCACTTTAG NGG 10 8 17 8 24 0 100 5 10
    240607012829_ydqa TTCACCTAAAGTGATTTAGCCTAGGCGAGGAG(+ATATAT)GATCCCTACTACGTGCTGGATTATGTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240607012827_zek4 CCTACATAAGCCCTTCATTGTGGGAGTAAATG(+ATATAT)GTACCGAAGCCCCAGAAAGTCCTACAGAGAT NGG 10 8 17 8 24 0 100 5 10
    240607012826_02f7 AAAAAATCTCTGTAGGACTTTCTGGGGCTTCG(+ATATAT)GTACCATTTACTCCCACAATGAAGGGCTTAT NGG 10 8 17 8 24 0 100 5 10
    240607012825_yvkl TGTGATCCCAACACAGGAAGTGGCAACAGGAG(+ATATAT)GATCCAGAGTTCAAAGTCAGCCTTGGATACA NGG 10 8 17 8 24 0 100 5 10
    240607012823_omjd GCATATGTATCCAAGGCTGACTTTGAACTCTG(+ATATAT)GATCCTCCTGTTGCCACTTCCTGTGTTGGGA NGG 10 8 17 8 24 0 100 5 10
    240607012822_88jp GCAATCGAGACAGAGAGAGCCAGCAAGGGCTG(+ATATAT)GTACCTGAGGTCATATTTCAGACACCCACCT NGG 10 8 17 8 24 0 100 5 10