Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607012820_j1rw GGGTCAGGTGGGTGTCTGAAATATGACCTCAG(+ATATAT)GTACCAGCCCTTGCTGGCTCTCTCTGTCTCG NGG 10 8 17 8 24 0 100 5 10
    240607012819_f5ch GCTACTACCTTGAGAACTGATAATGGAGCTTG(+ATATAT)GATCCAATTTAGATAGAAAAAATATTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240607012818_vva3 GAAAATAAAAAATATTTTTTCTATCTAAATTG(+ATATAT)GATCCAAGCTCCATTATCAGTTCTCAAGGTA NGG 10 8 17 8 24 0 100 5 10
    240607012816_zvu5 TGTATGTAACCCATGTCAATCAGATGCAGGTG(+ATATAT)GTACCCACACGACCAGAGTGATCAATAGGAA NGG 10 8 17 8 24 0 100 5 10
    240607012814_irmm GAAAATTCCTATTGATCACTCTGGTCGTGTGG(+ATATAT)GTACCACCTGCATCTGATTGACATGGGTTAC NGG 10 8 17 8 24 0 100 5 10
    240607012812_pri7 CCACAAGGCACGATATTTATATCCACCTAGGG(+ATATAT)GTACCGGCTTTTACTATTTAAAGCTATTTTT NGG 10 8 17 8 24 0 100 5 10
    240607012810_jnzd GTTTCAAAAATAGCTTTAAATAGTAAAAGCCG(+ATATAT)GTACCCCTAGGTGGATATAAATATCGTGCCT NGG 10 8 17 8 24 0 100 5 10
    240607012808_5whd TGGCTCTGCAGCCACATCGTGGCTATGTTCCG(+ATATAT)GATCCCATCCAGGGTGTGCATGTGCCAAGAA NGG 10 8 17 8 24 0 100 5 10
    240607012807_no1a CGCATTTCTTGGCACATGCACACCCTGGATGG(+ATATAT)GATCCGGAACATAGCCACGATGTGGCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240607012806_mhra GGACATTAACCTTTGGAAGTCTATATACAAAG(+ATATAT)GTACCATGGAGTAACTGACAGCTACCAAGTT NGG 10 8 17 8 24 0 100 5 10