Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613182036_wju4 CCTATGTAGTAACTGATTTCTGCACACTGGAG(+AGGACA)GTACCTGTTTGCCTTTAAAAGGTATGATAAA NGG 10 8 17 8 24 0 100 5 10
    240613182035_z52b TTTATTTTATCATACCTTTTAAAGGCAAACAG(+AGGACA)GTACCTCCAGTGTGCAGAAATCAGTTACTAC NGG 10 8 17 8 24 0 100 5 10
    240613182034_sy7l TTCCCAGGCATGACACCTGCCACTGTTGGCTG(+AGGACA)GATCCTCTAGGCATGGAGTGAGGAGGAGAGT NGG 10 8 17 8 24 0 100 5 10
    240613182032_votl TCCCCACTCTCCTCCTCACTCCATGCCTAGAG(+AGGACA)GATCCAGCCAACAGTGGCAGGTGTCATGCCT NGG 10 8 17 8 24 0 100 5 10
    240613182031_bn2o CTAGGATATGGGTGGGTCTCAGATTTCTGCAG(+AGGACA)GATCCATGACTGAAAAGCGAGTCTTATAACT NGG 10 8 17 8 24 0 100 5 10
    240613182030_op13 TGACCAGTTATAAGACTCGCTTTTCAGTCATG(+AGGACA)GATCCTGCAGAAATCTGAGACCCACCCATAT NGG 10 8 17 8 24 0 100 5 10
    240613182028_i7cv CAGGCACTTACTTCTTGCAGATGGTAGTCTGG(+AGGACA)GATCCTACACTGTTACAAGTGTTTTAACCTA NGG 10 8 17 8 24 0 100 5 10
    240613182027_qihy AGAGTTAGGTTAAAACACTTGTAACAGTGTAG(+AGGACA)GATCCCAGACTACCATCTGCAAGAAGTAAGT NGG 10 8 17 8 24 0 100 5 10
    240613182025_qjq8 GACGAGAAAAACAAAGATGTATTTAAATCCTG(+AGGACA)GATCCACTACCGCTGTTGTGATTTGGGAGTA NGG 10 8 17 8 24 0 100 5 10
    240613182024_twkq ACGTATACTCCCAAATCACAACAGCGGTAGTG(+AGGACA)GATCCAGGATTTAAATACATCTTTGTTTTTC NGG 10 8 17 8 24 0 100 5 10