Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613184836_xnxv GCCTTCTTCGTTGTCAACTCGGGGCACTCCTG(+AGGACA)GATCCTATGAGCCCCGGACAAGGTGCATTGT NGG 10 8 17 8 24 0 100 5 10
    240613184835_tf1x TTAGGGGAGAGAGTAAGGAGGATACTGATTAG(+AGGACA)GTACCAAGGTTTCATTACCTTAAAGGCAAAA NGG 10 8 17 8 24 0 100 5 10
    240613184833_i5tc CTGGTTTTTGCCTTTAAGGTAATGAAACCTTG(+AGGACA)GTACCTAATCAGTATCCTCCTTACTCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240613184831_wujp ATGGGACCTATTCCAGCCCCTTGAATCTTAAG(+AGGACA)GTACCATATGAGAGCTAAGAGCAGGTTTCCC NGG 10 8 17 8 24 0 100 5 10
    240613184830_6bac ATGCTGGGAAACCTGCTCTTAGCTCTCATATG(+AGGACA)GTACCTTAAGATTCAAGGGGCTGGAATAGGT NGG 10 8 17 8 24 0 100 5 10
    240613184828_xyth CTTGCTCTCCAGGAGCTCACAGTTCAAGCTGG(+AGGACA)GATCCAGATGAGCAAACATGATCATCACACA NGG 10 8 17 8 24 0 100 5 10
    240613184827_ajxu TATACTGTGTGATGATCATGTTTGCTCATCTG(+AGGACA)GATCCCAGCTTGAACTGTGAGCTCCTGGAGA NGG 10 8 17 8 24 0 100 5 10
    240613184825_5595 GGTGTCAGAGTTTGGATGCTGATTATGGGGTG(+AGGACA)GATCCCTGGATATGGCAGTCTCTACATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613184823_yhpa GGATGGACCATGTAGAGACTGCCATATCCAGG(+AGGACA)GATCCACCCCATAATCAGCATCCAAACTCTG NGG 10 8 17 8 24 0 100 5 10
    240613184821_cqp0 ACGGACTGCTAAATTGGGTCCTATAGATCGAG(+AGGACA)GTACCATAATTAAGATATTTTAATAAGGCTC NGG 10 8 17 8 24 0 100 5 10