Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613184852_8m6s GTTATGTGATAATTTTAATCTTGGAATGAATG(+AGGACA)GATCCAATATGTAGATTGTTCATTTTATAGT NGG 10 8 17 8 24 0 100 5 10
    240613184851_6ae5 ACTTGCATGAGTTGATTCTCTCTTACCGCGTG(+AGGACA)GATCCCTGGGATTGAATTCAGGCCACCAGGC NGG 10 8 17 8 24 0 100 5 10
    240613184848_k0yn CACGAGCCTGGTGGCCTGAATTCAATCCCAGG(+AGGACA)GATCCACGCGGTAAGAGAGAATCAACTCATG NGG 10 8 17 8 24 0 100 5 10
    240613184847_f9ps ACCTCCAAGAAGCAGGCTTGATGGTCAGAAAG(+AGGACA)GATCCTTGAGGCCAAGGAAGGATCTTTGTTG NGG 10 8 17 8 24 0 100 5 10
    240613184846_e1uc GACCCCAACAAAGATCCTTCCTTGGCCTCAAG(+AGGACA)GATCCTTTCTGACCATCAAGCCTGCTTCTTG NGG 10 8 17 8 24 0 100 5 10
    240613184844_ez9g AGGGGGGCATGCAATATGGGTGAGGGAGGGCG(+AGGACA)GTACCCTTCTCATCCCAGACCTGAGCCCCCC NGG 10 8 17 8 24 0 100 5 10
    240613184842_o4d9 GGGAGGGGGGGCTCAGGTCTGGGATGAGAAGG(+AGGACA)GTACCGCCCTCCCTCACCCATATTGCATGCC NGG 10 8 17 8 24 0 100 5 10
    240613184840_jngv AGGAGAGAAGATGGACACAAGGGCAGATGAGG(+AGGACA)GATCCAGACAAGAAGATAGAGTTCCTGCTAT NGG 10 8 17 8 24 0 100 5 10
    240613184839_1rdn GAACAATAGCAGGAACTCTATCTTCTTGTCTG(+AGGACA)GATCCCTCATCTGCCCTTGTGTCCATCTTCT NGG 10 8 17 8 24 0 100 5 10
    240613184838_5j5e GCAGGACAATGCACCTTGTCCGGGGCTCATAG(+AGGACA)GATCCAGGAGTGCCCCGAGTTGACAACGAAG NGG 10 8 17 8 24 0 100 5 10