Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613183228_3js2 CAAAAGACAAAGATGCCTTTTCAAAATGGCGG(+AGGACA)GATCCACCTGGAGGAAATCCTAGTGAAACCA NGG 10 8 17 8 24 0 100 5 10
    240613183226_ebgp GGAAGTGGTTTCACTAGGATTTCCTCCAGGTG(+AGGACA)GATCCCGCCATTTTGAAAAGGCATCTTTGTC NGG 10 8 17 8 24 0 100 5 10
    240613183225_u3gv TGCTGGAAGCCCTGGGTTTCACCTCCAGAACG(+AGGACA)GTACCTGTGATCACAACACTCAAGAGGTAGA NGG 10 8 17 8 24 0 100 5 10
    240613183224_j3t7 CTGCCTCTACCTCTTGAGTGTTGTGATCACAG(+AGGACA)GTACCGTTCTGGAGGTGAAACCCAGGGCTTC NGG 10 8 17 8 24 0 100 5 10
    240613183222_3avy TCATGACTACAGCTCTGGGTCACCTCCTGTGG(+AGGACA)GATCCACCAGGGGACGGGTTACATCTTCCTG NGG 10 8 17 8 24 0 100 5 10
    240613183221_zn9k GGCTTCAGGAAGATGTAACCCGTCCCCTGGTG(+AGGACA)GATCCCACAGGAGGTGACCCAGAGCTGTAGT NGG 10 8 17 8 24 0 100 5 10
    240613183219_0gtk CAAAAGACAAAGATGCCTTTTCAAAATGGCGG(+AGGACA)GATCCACCTGGAGGAAATCCTAGTGAAACCA NGG 10 8 17 8 24 0 100 5 10
    240613183217_ywl5 GGAAGTGGTTTCACTAGGATTTCCTCCAGGTG(+AGGACA)GATCCCGCCATTTTGAAAAGGCATCTTTGTC NGG 10 8 17 8 24 0 100 5 10
    240613183216_eida TGTTTTTTCCTTTGTTGACTTCACTTGTAGTG(+AGGACA)GTACCATTTTTGGGATGTGTACTGTTTCTAT NGG 10 8 17 8 24 0 100 5 10
    240613183215_l3ni TTCCCATAGAAACAGTACACATCCCAAAAATG(+AGGACA)GTACCACTACAAGTGAAGTCAACAAAGGAAA NGG 10 8 17 8 24 0 100 5 10