Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607005341_8xns GTTCCAGGCTTTGGAGGGGTTTGATTGGTTGG(+ATATAT)GTACCCAACCCCAGTCTACTAAGTTCCAGAC NGG 10 8 17 8 24 0 100 5 10
    240607005338_6q82 TGTGAACGAGACAATGGAGCAAGGGGGAGGGG(+ATATAT)GTACCTTCCATTTCCCAGCAGTTGTTCCAGT NGG 10 8 17 8 24 0 100 5 10
    240607005337_x5sz ACCAGACTGGAACAACTGCTGGGAAATGGAAG(+ATATAT)GTACCCCCTCCCCCTTGCTCCATTGTCTCGT NGG 10 8 17 8 24 0 100 5 10
    240607005335_hv0q ATCAGAAGACACCCTCAGGTGTCACTCCTTAG(+ATATAT)GTACCACCCACTTACAGTTTTGAGATGAGTT NGG 10 8 17 8 24 0 100 5 10
    240607005333_simz GAGAGAACTCATCTCAAAACTGTAAGTGGGTG(+ATATAT)GTACCTAAGGAGTGACACCTGAGGGTGTCTT NGG 10 8 17 8 24 0 100 5 10
    240607005332_i1o3 TAGTAATGGTAACCCTGACTAAGACAGAACTG(+ATATAT)GTACCTGGGGCATTGCTGTGACAGACCTGAC NGG 10 8 17 8 24 0 100 5 10
    240607005329_qxrx GCATTGTCAGGTCTGTCACAGCAATGCCCCAG(+ATATAT)GTACCAGTTCTGTCTTAGTCAGGGTTACCAT NGG 10 8 17 8 24 0 100 5 10
    240607005328_l0w8 TGAGCTTCCAGTCTGTAGAGATGGGGAGGAGG(+ATATAT)GTACCCTGTGTTCATTTGAGCTCACCACTGT NGG 10 8 17 8 24 0 100 5 10
    240607005326_zz6n CCTGTACAGTGGTGAGCTCAAATGAACACAGG(+ATATAT)GTACCCTCCTCCCCATCTCTACAGACTGGAA NGG 10 8 17 8 24 0 100 5 10
    240607005325_4a7e TTATTAATGGCCTCCCAGTCGTGCTATGAAAG(+ATATAT)GATCCATTATTGCCCAGAGCAAGATGGCCAT NGG 10 8 17 8 24 0 100 5 10