Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607005240_4tzw GCACACGGCTTTGGGAATACGAAGTGTCTTCG(+ATATAT)GTACCAGGTCTGTGTTTCGCTTTGGTTTGGG NGG 10 8 17 8 24 0 100 5 10
    240607005238_o0c9 GTTTGTCTGGGTGTAGGGTTGGACAGCTCTGG(+ATATAT)GATCCGATTAAGGCCATGTTCTGGGGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240607005237_zas7 TGAGGGGCAGCCCCAGAACATGGCCTTAATCG(+ATATAT)GATCCCAGAGCTGTCCAACCCTACACCCAGA NGG 10 8 17 8 24 0 100 5 10
    240607005236_7xad ATGCTGCCTGTGAAGCTGTGTTCTCAGGTCAG(+ATATAT)GATCCTGTTCACCAGCGTCAGAAAGTGTACA NGG 10 8 17 8 24 0 100 5 10
    240607005235_erc4 TGGTGTGTACACTTTCTGACGCTGGTGAACAG(+ATATAT)GATCCTGACCTGAGAACACAGCTTCACAGGC NGG 10 8 17 8 24 0 100 5 10
    240607005234_mjnr CACGCGCCGCTCTGCTCCGGAGCTCTGCGAAG(+ATATAT)GATCCCGACCGCGCCGGGCTATGCAGGCAGC NGG 10 8 17 8 24 0 100 5 10
    240607005232_cxrz CTGCAGCTGCCTGCATAGCCCGGCGCGGTCGG(+ATATAT)GATCCTTCGCAGAGCTCCGGAGCAGAGCGGC NGG 10 8 17 8 24 0 100 5 10
    240607005230_4ed6 AGTCTCCAGATTCAGGTAACAGCTTGATATGG(+ATATAT)GATCCATAAGTGTCAAATTGGGCTTTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240607005229_2iqx TTTTTCACACAAAGCCCAATTTGACACTTATG(+ATATAT)GATCCCATATCAAGCTGTTACCTGAATCTGG NGG 10 8 17 8 24 0 100 5 10
    240607005228_gxip TCTCTTATGAAGTCAGGCCGGCCAGGGAAGGG(+ATATAT)GATCCCATAGATTCCTATGAAAGCTATCAAG NGG 10 8 17 8 24 0 100 5 10