Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607005154_2n2u GGTGTTATGGGCTGCTCTTGCAGAGCATTGAG(+ATATAT)GATCCTCCAGTCCCAATGAGTTTGATGGTCT NGG 10 8 17 8 24 0 100 5 10
    240607005153_q6fj CTGCCTTGTGAAAAAATTTTCATGCCAAGTTG(+ATATAT)GTACCTCTGAGAATAATTGGTGACTAAAAGG NGG 10 8 17 8 24 0 100 5 10
    240607005152_hamr ATACACCTTTTAGTCACCAATTATTCTCAGAG(+ATATAT)GTACCAACTTGGCATGAAAATTTTTTCACAA NGG 10 8 17 8 24 0 100 5 10
    240607005150_mkl7 TGACTTGCCAGAAATCGTGTTAGTCAGATGTG(+ATATAT)GTACCACATGCCCATAACCCGAAAACTTGAG NGG 10 8 17 8 24 0 100 5 10
    240607005149_d570 TATCTCTCAAGTTTTCGGGTTATGGGCATGTG(+ATATAT)GTACCACATCTGACTAACACGATTTCTGGCA NGG 10 8 17 8 24 0 100 5 10
    240607005147_9qz3 AAATTAAAGTCAACCAGTAGAGTGAGAGAGGG(+ATATAT)GATCCAGAGACATCCATGCCTACATCTGTGA NGG 10 8 17 8 24 0 100 5 10
    240607005146_hx8q TGAGTTCACAGATGTAGGCATGGATGTCTCTG(+ATATAT)GATCCCCTCTCTCACTCTACTGGTTGACTTT NGG 10 8 17 8 24 0 100 5 10
    240607005145_k50x ACATGTCAGTGTAGTGTTCACAGAGGGAGCTG(+ATATAT)GATCCCCTGGAACTGGAATCGCAAGCAATTG NGG 10 8 17 8 24 0 100 5 10
    240607005142_23de GCTCACAATTGCTTGCGATTCCAGTTCCAGGG(+ATATAT)GATCCAGCTCCCTCTGTGAACACTACACTGA NGG 10 8 17 8 24 0 100 5 10
    240607005141_4ohr CTGGAGTGGGAGGAGAATTCACATCCTCGGAG(+ATATAT)GATCCAGAGAGGCCCAGCGACCATGCTGCAC NGG 10 8 17 8 24 0 100 5 10