Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607005140_ygp7 AAGATGTGCAGCATGGTCGCTGGGCCTCTCTG(+ATATAT)GATCCTCCGAGGATGTGAATTCTCCTCCCAC NGG 10 8 17 8 24 0 100 5 10
    240607005138_2fqj TTTGGCTTCTTTGGAGATGTCAGAGGCCTCAG(+ATATAT)GATCCAGTTCCCGGCGTCTTTGAGCCCAGCC NGG 10 8 17 8 24 0 100 5 10
    240607005137_aplj TGGCTGGCTGGGCTCAAAGACGCCGGGAACTG(+ATATAT)GATCCTGAGGCCTCTGACATCTCCAAAGAAG NGG 10 8 17 8 24 0 100 5 10
    240607005136_cesc GCAGCCCCCAAGAATAGCATCTATACAGGCTG(+ATATAT)GTACCCCAGGTTTCCACAGGAAACAGCTGCT NGG 10 8 17 8 24 0 100 5 10
    240607005133_23jq CTGCCAGCAGCTGTTTCCTGTGGAAACCTGGG(+ATATAT)GTACCAGCCTGTATAGATGCTATTCTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240607005131_7u9s CAGGCGGGCCTCTTTCCTCTCATTTAACCCAG(+ATATAT)GATCCTTAGGCTGAAACAGTTCCCCAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240607005129_gbbc CTGCTCTGCTTGGGGAACTGTTTCAGCCTAAG(+ATATAT)GATCCTGGGTTAAATGAGAGGAAAGAGGCCC NGG 10 8 17 8 24 0 100 5 10
    240607005128_0r8g AAGAGAGCTAGGGGTGGGACAGTCCAAACTGG(+ATATAT)GTACCCATAGCCCAGTCTCGGTCAAGCTGGT NGG 10 8 17 8 24 0 100 5 10
    240607005126_okln AGGGGACCAGCTTGACCGAGACTGGGCTATGG(+ATATAT)GTACCCAGTTTGGACTGTCCCACCCCTAGCT NGG 10 8 17 8 24 0 100 5 10
    240607005125_9xut CTGGCTTGTAGCGCCCCCTGTCTTGACACTCG(+ATATAT)GATCCCCAATTGCTCCTCTGTTTTCCCTTTT NGG 10 8 17 8 24 0 100 5 10