Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607005108_5d7u CAGGGACCCAGGGCTTGACCCTGGGTATGTGG(+ATATAT)GATCCAGAAGGAGGTAGACCAACGGGGAAAG NGG 10 8 17 8 24 0 100 5 10
    240607005106_de1i ACATGTATATCTCTACACCATGATTGGGCCTG(+ATATAT)GTACCCAAAGGAGGACAGATGAGAGAATCAG NGG 10 8 17 8 24 0 100 5 10
    240607005104_jmzs GGGCTCTGATTCTCTCATCTGTCCTCCTTTGG(+ATATAT)GTACCAGGCCCAATCATGGTGTAGAGATATA NGG 10 8 17 8 24 0 100 5 10
    240607005103_2lft ACTGAAAGGTTTAAGAGGAAATGGGGTGATTG(+ATATAT)GTACCAAGCAGCAGGAGGTAGACTTCAGTTT NGG 10 8 17 8 24 0 100 5 10
    240607005102_ltnx GTAGCAAACTGAAGTCTACCTCCTGCTGCTTG(+ATATAT)GTACCAATCACCCCATTTCCTCTTAAACCTT NGG 10 8 17 8 24 0 100 5 10
    240607005100_w1e1 ATGACAGGCCCAAAACCTCCACAGGAGCCAGG(+ATATAT)GTACCAGGAGACATGAGAGAACTCCTTCTGT NGG 10 8 17 8 24 0 100 5 10
    240607005058_2mk0 ATGGGACAGAAGGAGTTCTCTCATGTCTCCTG(+ATATAT)GTACCCTGGCTCCTGTGGAGGTTTTGGGCCT NGG 10 8 17 8 24 0 100 5 10
    240607005056_90va ACAGCTTTGCATGTGTTGCCCTGACCTCAGAG(+ATATAT)GATCCTTCTTCTGTAGCCTCCAGGGGACTGA NGG 10 8 17 8 24 0 100 5 10
    240607005055_dq2e GAGTCTCAGTCCCCTGGAGGCTACAGAAGAAG(+ATATAT)GATCCTCTGAGGTCAGGGCAACACATGCAAA NGG 10 8 17 8 24 0 100 5 10
    240607005053_pw0f CTATATCCCTGGACCCTAGACAAGAGGACCCG(+ATATAT)GATCCACCTGTGGGCTTCTAGCATTACCCAG NGG 10 8 17 8 24 0 100 5 10