Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607005211_358q GTGTGATCTTGATTGCCTTCCTGAGACACCAG(+ATATAT)GTACCTGTTTTCCTTAATAGGTTATGAAGTA NGG 10 8 17 8 24 0 100 5 10
    240607005209_f9v7 CTGGAAGGAGTTAGCTCCCAGAGCTCCCCAGG(+ATATAT)GTACCCTCCCGGGGTCGGCCTTACCTTCCAT NGG 10 8 17 8 24 0 100 5 10
    240607005206_jnz1 TGGAGATGGAAGGTAAGGCCGACCCCGGGAGG(+ATATAT)GTACCCTGGGGAGCTCTGGGAGCTAACTCCT NGG 10 8 17 8 24 0 100 5 10
    240607005204_2f4t TTGAGTTATGGATCGTTAGATCTTCATCCTGG(+ATATAT)GATCCAGTTCCATTTTCTCTTCCGTCCCTGA NGG 10 8 17 8 24 0 100 5 10
    240607005202_uvhj TATCCTCAGGGACGGAAGAGAAAATGGAACTG(+ATATAT)GATCCCAGGATGAAGATCTAACGATCCATAA NGG 10 8 17 8 24 0 100 5 10
    240607005201_e3bc GACAGTCCATCCATCCTTAACTGCAGAATCTG(+ATATAT)GATCCCCTGCCCTCCAGTTTTGCTGCCGTTT NGG 10 8 17 8 24 0 100 5 10
    240607005159_lzmz TTGATAAACGGCAGCAAAACTGGAGGGCAGGG(+ATATAT)GATCCAGATTCTGCAGTTAAGGATGGATGGA NGG 10 8 17 8 24 0 100 5 10
    240607005158_uz1r ATATGTGTGTGTGTGTGTGTGTGTGTATATAG(+ATATAT)GTACCTACAATCTTTGTGGAATAGATTCTAA NGG 10 8 17 8 24 0 100 5 10
    240607005157_0w4r TATGTTTAGAATCTATTCCACAAAGATTGTAG(+ATATAT)GTACCTATATACACACACACACACACACACA NGG 10 8 17 8 24 0 100 5 10
    240607005155_ytce GGAAGAGACCATCAAACTCATTGGGACTGGAG(+ATATAT)GATCCTCAATGCTCTGCAAGAGCAGCCCATA NGG 10 8 17 8 24 0 100 5 10