Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607005310_ygwt AATTCTTGCCAGATTCATCACTTGGCCTTTGG(+ATATAT)GTACCCAAAGAATAAAAACAAGTTCTAAGAA NGG 10 8 17 8 24 0 100 5 10
    240607005308_ixlu TGTGGGAGAAGAGCCCTGACCAAGCTGATCCG(+ATATAT)GTACCTTGAAGGAAGTGCAATTGTTTCATGC NGG 10 8 17 8 24 0 100 5 10
    240607005307_wd9d AGTATGCATGAAACAATTGCACTTCCTTCAAG(+ATATAT)GTACCGGATCAGCTTGGTCAGGGCTCTTCTC NGG 10 8 17 8 24 0 100 5 10
    240607005306_ulon AGCTAGAGTCTGGGTTGGAGAGGCCGAGTGAG(+ATATAT)GATCCTTACAAGGCTACTGCATAGCTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240607005305_s1qf TCCCGTGAGAAGCTATGCAGTAGCCTTGTAAG(+ATATAT)GATCCTCACTCGGCCTCTCCAACCCAGACTC NGG 10 8 17 8 24 0 100 5 10
    240607005303_t37y CACAGAAGTTATCTTGACCAAATGGCCTATTG(+ATATAT)GTACCTACTCTTAATTTATTCTTTCAAGCAC NGG 10 8 17 8 24 0 100 5 10
    240607005302_yfp1 GGGGAGTGCTTGAAAGAATAAATTAAGAGTAG(+ATATAT)GTACCAATAGGCCATTTGGTCAAGATAACTT NGG 10 8 17 8 24 0 100 5 10
    240607005301_lf2a AAATAGCTCCCCACCTTCCCAAGGCTGTTATG(+ATATAT)GTACCAGACACACAGCTCAGGACACCCCAGG NGG 10 8 17 8 24 0 100 5 10
    240607005300_empr TTCTCCCTGGGGTGTCCTGAGCTGTGTGTCTG(+ATATAT)GTACCATAACAGCCTTGGGAAGGTGGGGAGC NGG 10 8 17 8 24 0 100 5 10
    240607005258_sooj CTCCATTACACCAAGGACACAGAAAAACACAG(+ATATAT)GTACCGATTTGCCTTCCTCTCCTGCCAATCA NGG 10 8 17 8 24 0 100 5 10