Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607004959_he7j ATATTTTCTTATAAGGACATTCATATCCAAAG(+ATATAT)GTACCAATATCCCAGATAGGTACTAACTGGA NGG 10 8 17 8 24 0 100 5 10
    240607004957_dwid TGAAACTGGACTTGCTGGGGTGTCGCTAAGAG(+ATATAT)GATCCCATTTGACCTCTGCCTTGCCACAGCC NGG 10 8 17 8 24 0 100 5 10
    240607004956_1pe0 TCTCAGGCTGTGGCAAGGCAGAGGTCAAATGG(+ATATAT)GATCCTCTTAGCGACACCCCAGCAAGTCCAG NGG 10 8 17 8 24 0 100 5 10
    240607004955_vfpk CTTTGCTGCCTAAGAACCGGAGCCAACAGCAG(+ATATAT)GTACCTCCTGGGGTTCCCTGAGGAGTGTGCT NGG 10 8 17 8 24 0 100 5 10
    240607004953_w5it GTTTTAGCACACTCCTCAGGGAACCCCAGGAG(+ATATAT)GTACCTGCTGTTGGCTCCGGTTCTTAGGCAG NGG 10 8 17 8 24 0 100 5 10
    240607004951_av9e GTAGGGTCCCAAAGGAGAGCCGGGGCAGGGTG(+ATATAT)GATCCCTGCTTTGCTGCCTAAGAACCGGAGC NGG 10 8 17 8 24 0 100 5 10
    240607004950_dvmt TGTTGGCTCCGGTTCTTAGGCAGCAAAGCAGG(+ATATAT)GATCCACCCTGCCCCGGCTCTCCTTTGGGAC NGG 10 8 17 8 24 0 100 5 10
    240607004948_c1ab GGCAGAGGGACCTATGGCCAGATGCCAGGGAG(+ATATAT)GTACCCAGAGACCCCACAGCGTGGTTCATGC NGG 10 8 17 8 24 0 100 5 10
    240607004946_htly GATCTGCATGAACCACGCTGTGGGGTCTCTGG(+ATATAT)GTACCTCCCTGGCATCTGGCCATAGGTCCCT NGG 10 8 17 8 24 0 100 5 10
    240607004945_o466 GGTGACAGCTCAGTGTATTAGTGCTGGGTGTG(+ATATAT)GTACCTGCTCTGGAGTCAGACTGCCTAGCAT NGG 10 8 17 8 24 0 100 5 10