Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607004913_c7c2 ACAGAAACAAGCAGCATCAGGGAAGGAAAATG(+ATATAT)GTACCAACTCAGGCTTCAGTTTGGAATAAAA NGG 10 8 17 8 24 0 100 5 10
    240607004912_nee7 GGGGTGAGGCTGAGCGCTATACATGCAGTGTG(+ATATAT)GATCCTGGTGCCAGTCTTCCTTTTGACTGCT NGG 10 8 17 8 24 0 100 5 10
    240607004910_t2e9 TGGAAAGCAGTCAAAAGGAAGACTGGCACCAG(+ATATAT)GATCCACACTGCATGTATAGCGCTCAGCCTC NGG 10 8 17 8 24 0 100 5 10
    240607004909_7i53 TATCATTTTATTTTTGAAGGCAGTTAGGAGTG(+ATATAT)GATCCTCTTTTTCCTGCATATCATGTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240607004908_llcx GTTTTTAAAAACATGATATGCAGGAAAAAGAG(+ATATAT)GATCCACTCCTAACTGCCTTCAAAAATAAAA NGG 10 8 17 8 24 0 100 5 10
    240607004907_waqh AATGCAGACAACTGACTCTAGATGTCAGTGTG(+ATATAT)GTACCAAAGAACTGCAGCCCTAGTGAGCTTG NGG 10 8 17 8 24 0 100 5 10
    240607004905_2h7s CCTTTCAAGCTCACTAGGGCTGCAGTTCTTTG(+ATATAT)GTACCACACTGACATCTAGAGTCAGTTGTCT NGG 10 8 17 8 24 0 100 5 10
    240607004904_q89i CGTTTGGCCTGCATGGTTGTGGGAGAGCATCG(+ATATAT)GATCCTTAAAACTGGAATTACAGACAGGTGT NGG 10 8 17 8 24 0 100 5 10
    240607004902_sn6m AGCTCACACCTGTCTGTAATTCCAGTTTTAAG(+ATATAT)GATCCGATGCTCTCCCACAACCATGCAGGCC NGG 10 8 17 8 24 0 100 5 10
    240607004901_u4py TTGGCTGCGTCTTTCGCCAAGCTAGGCTACTG(+ATATAT)GTACCTGCCTTGGGCCGTGGATGGGGTGATA NGG 10 8 17 8 24 0 100 5 10