Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607004841_1qyp GGATGGACTTTCCAGAGACTACCCCACCCGGG(+ATATAT)GATCCATCCCATAATCAGCCATCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240607004839_mc3r AAACTGTCCAAAGCACTGGACTGACATCACTG(+ATATAT)GTACCAGGGACGCCTACCTTTCTGGGGTATT NGG 10 8 17 8 24 0 100 5 10
    240607004837_k1t2 TTACAAATACCCCAGAAAGGTAGGCGTCCCTG(+ATATAT)GTACCAGTGATGTCAGTCCAGTGCTTTGGAC NGG 10 8 17 8 24 0 100 5 10
    240607004835_d5mx GTTCTTATAAAGCCACTAATCCTATCATGAGG(+ATATAT)GATCCACCTTCATGAACTCCACTTATCCTGG NGG 10 8 17 8 24 0 100 5 10
    240607004833_fay4 CTTATCCAGGATAAGTGGAGTTCATGAAGGTG(+ATATAT)GATCCCTCATGATAGGATTAGTGGCTTTATA NGG 10 8 17 8 24 0 100 5 10
    240607004832_uxo4 GAACTTTAGCTTCTCCGAAGCCAGGGAGCAAG(+ATATAT)GATCCCAGTTCCAGGTCCTGACATCAGTTTC NGG 10 8 17 8 24 0 100 5 10
    240607004830_wemy ACTCAGAAACTGATGTCAGGACCTGGAACTGG(+ATATAT)GATCCTTGCTCCCTGGCTTCGGAGAAGCTAA NGG 10 8 17 8 24 0 100 5 10
    240607004829_78xx TAAAGACCACAGTAGAGAAGTAGTTAGTGCTG(+ATATAT)GTACCATCCACAGCCACTAGGAACTAACCAC NGG 10 8 17 8 24 0 100 5 10
    240607004828_kbr3 CATGAGTGGTTAGTTCCTAGTGGCTGTGGATG(+ATATAT)GTACCAGCACTAACTACTTCTCTACTGTGGT NGG 10 8 17 8 24 0 100 5 10
    240607004827_rkxv CTCAGTGCTGAAAGACTTGATTTTGTAGCTTG(+ATATAT)GTACCTCAGCGTTGACTTAGGTGCTCCACTA NGG 10 8 17 8 24 0 100 5 10