Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607004754_1fqt TAAAGGCAAACCTGAGAAATGAAGAATCATAG(+ATATAT)GTACCCTCTATGGTTGGCAACCGGGTCACTC NGG 10 8 17 8 24 0 100 5 10
    240607004753_k3by AATAAATAAAGTGTAGTGATTCATTAAATATG(+ATATAT)GTACCTACAGGATCAAAAATACCAGTACTCC NGG 10 8 17 8 24 0 100 5 10
    240607004752_ovi5 TGATTGGAGTACTGGTATTTTTGATCCTGTAG(+ATATAT)GTACCATATTTAATGAATCACTACACTTTAT NGG 10 8 17 8 24 0 100 5 10
    240607004750_xfn8 TGCTTAGGAGTGAGAACTGCCAGGGTAGCCAG(+ATATAT)GATCCTCTCACAGTATTAAGGCGTGCCCACA NGG 10 8 17 8 24 0 100 5 10
    240607004749_lckp TCCCATGTGGGCACGCCTTAATACTGTGAGAG(+ATATAT)GATCCTGGCTACCCTGGCAGTTCTCACTCCT NGG 10 8 17 8 24 0 100 5 10
    240607004747_njcl GCCCTCATCTCTGCTACCGTTTACATCATCCG(+ATATAT)GTACCTTTGTAGCCTTAGACCGATGATCACC NGG 10 8 17 8 24 0 100 5 10
    240607004746_jrfw AACACGGTGATCATCGGTCTAAGGCTACAAAG(+ATATAT)GTACCGGATGATGTAAACGGTAGCAGAGATG NGG 10 8 17 8 24 0 100 5 10
    240607004745_clyj CCAACACTTAGAACATTTTTTTAAACAGAAAG(+ATATAT)GTACCAGTACCTAGACACAAACGTTAATGAG NGG 10 8 17 8 24 0 100 5 10
    240607004743_67zd TCGTACTCATTAACGTTTGTGTCTAGGTACTG(+ATATAT)GTACCTTTCTGTTTAAAAAAATGTTCTAAGT NGG 10 8 17 8 24 0 100 5 10
    240607004742_axm5 CCAGAGCATATACAAAATTATACAAATTACAG(+ATATAT)GTACCTGGTTTGTAAAACTGACCATCAGGTG NGG 10 8 17 8 24 0 100 5 10