Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607004726_vbbc AATGACTCTCTCCCACAGGTTTGCCATGCTAG(+ATATAT)GTACCTAGACAAGAAATGGTAAGACAGAGTT NGG 10 8 17 8 24 0 100 5 10
    240607004725_q8gx GATTTTGGCCTTTTTCAGTTGGGTACTATATG(+ATATAT)GTACCAGGATGAATACATGGCCTTTAATGTT NGG 10 8 17 8 24 0 100 5 10
    240607004723_h0qv GAAGGAACATTAAAGGCCATGTATTCATCCTG(+ATATAT)GTACCATATAGTACCCAACTGAAAAAGGCCA NGG 10 8 17 8 24 0 100 5 10
    240607004722_3re7 GCTTGCTTCATAAGCATGAAGACTGCCATTTG(+ATATAT)GATCCTAGTACCCAAACAAAAGCTGGGCATT NGG 10 8 17 8 24 0 100 5 10
    240607004721_w2s7 GGGACAATGCCCAGCTTTTGTTTGGGTACTAG(+ATATAT)GATCCAAATGGCAGTCTTCATGCTTATGAAG NGG 10 8 17 8 24 0 100 5 10
    240607004720_jc2i GGACAGCTCACCATTATGATCTGTACCAGTTG(+ATATAT)GTACCTGCCTTGGTCAGCAGGGAGCTGAGGA NGG 10 8 17 8 24 0 100 5 10
    240607004718_kdis TATGATCCTCAGCTCCCTGCTGACCAAGGCAG(+ATATAT)GTACCAACTGGTACAGATCATAATGGTGAGC NGG 10 8 17 8 24 0 100 5 10
    240607004717_bmq6 GTTCACTAAGTAACTTGTGCTCATTTTCATTG(+ATATAT)GATCCTGCAGTGACTCATTTCCATATGGTCG NGG 10 8 17 8 24 0 100 5 10
    240607004716_3egl CAATGCGACCATATGGAAATGAGTCACTGCAG(+ATATAT)GATCCAATGAAAATGAGCACAAGTTACTTAG NGG 10 8 17 8 24 0 100 5 10
    240607004714_2ytm GCTACATCTCCCAGACTTTCAGTCTTATCTAG(+ATATAT)GATCCTCTCCTCTTCCCAGCCTGAACCCAGG NGG 10 8 17 8 24 0 100 5 10