Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607004644_dxk6 TATTACTGATGCCATGTTGTGCTTGCAGATAG(+ATATAT)GATCCTAGTATGGCTGTCCTCTAAGAGACTC NGG 10 8 17 8 24 0 100 5 10
    240607004642_vjlh ATCAAATAGTTAAGAAGGAAATATTTCAAATG(+ATATAT)GTACCAAATAAGACTCCTTTGTAAGCTTAGA NGG 10 8 17 8 24 0 100 5 10
    240607004641_8vbl AAAAATCTAAGCTTACAAAGGAGTCTTATTTG(+ATATAT)GTACCATTTGAAATATTTCCTTCTTAACTAT NGG 10 8 17 8 24 0 100 5 10
    240607004639_474i ATCAAATAGTTAAGAAGGAAATATTTCAAATG(+ATATAT)GTACCAAATAAGACTCCTTTGTAAGCTTAGA NGG 10 8 17 8 24 0 100 5 10
    240607004638_j0ch AAAAATCTAAGCTTACAAAGGAGTCTTATTTG(+ATATAT)GTACCATTTGAAATATTTCCTTCTTAACTAT NGG 10 8 17 8 24 0 100 5 10
    240607004637_4a6o TTTAGTCACAATAAAGACCCCAAATGAATAAG(+ATATAT)GTACCATGTGTGTATATATATACACATATAT NGG 10 8 17 8 24 0 100 5 10
    240607004636_kmlc TGTGTATATATGTGTATATATATACACACATG(+ATATAT)GTACCTTATTCATTTGGGGTCTTTATTGTGA NGG 10 8 17 8 24 0 100 5 10
    240607004635_8snw TTTAGTCACAATAAAGACCCCAAATGAATAAG(+ATATAT)GTACCATGTGTGTATATATATACACATATAT NGG 10 8 17 8 24 0 100 5 10
    240607004634_dl9y TGTGTATATATGTGTATATATATACACACATG(+ATATAT)GTACCTTATTCATTTGGGGTCTTTATTGTGA NGG 10 8 17 8 24 0 100 5 10
    240607004632_mez2 TTTAGTCACAATAAGGACCCCAAATGAATAAG(+ATATAT)GTACCATGTGTGTGTGTGTGTGTGTGTATAC NGG 10 8 17 8 24 0 100 5 10