Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607004631_zhen TATGTGTATACACACACACACACACACACATG(+ATATAT)GTACCTTATTCATTTGGGGTCCTTATTGTGA NGG 10 8 17 8 24 0 100 5 10
    240607004630_rbnt TAGTGTTTAAAGCATGTGATGCAGTGGCAGAG(+ATATAT)GATCCAGGTTTAGTTCCTAGCACCTGTGTGG NGG 10 8 17 8 24 0 100 5 10
    240607004628_89ta AGGTGCCACACAGGTGCTAGGAACTAAACCTG(+ATATAT)GATCCTCTGCCACTGCATCACATGCTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240607004626_aanx GTGGTAAGTGATTAACCATGTTCTGGTGGCGG(+ATATAT)GTACCAAACTGTTCTTCATGATAGAGTAGCT NGG 10 8 17 8 24 0 100 5 10
    240607004625_89bw GAAGCAGCTACTCTATCATGAAGAACAGTTTG(+ATATAT)GTACCCGCCACCAGAACATGGTTAATCACTT NGG 10 8 17 8 24 0 100 5 10
    240607004624_31ri TCATAGTGGTCCACTGAGGTCATATATTTCTG(+ATATAT)GATCCTTCCGTCTTAGGAAATATCAACCCTC NGG 10 8 17 8 24 0 100 5 10
    240607004622_6s2o CAGAAGAGGGTTGATATTTCCTAAGACGGAAG(+ATATAT)GATCCAGAAATATATGACCTCAGTGGACCAC NGG 10 8 17 8 24 0 100 5 10
    240607004620_587g CAGAGTTCCCTGTAGATGGACTGATTCACCAG(+ATATAT)GATCCTGCTACACTGAAGGCGCACAGTTGAC NGG 10 8 17 8 24 0 100 5 10
    240607004619_0jzr GGCTGGTCAACTGTGCGCCTTCAGTGTAGCAG(+ATATAT)GATCCTGGTGAATCAGTCCATCTACAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240607004618_r1s0 GTGATTAGAGTAGGTGGTCGGGGCCACACTAG(+ATATAT)GTACCTAACCTACACACAAGCACACAGAATC NGG 10 8 17 8 24 0 100 5 10