Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607004658_c73z GTGAGTTCCAGGACAGCCAAGACTACACAGAG(+ATATAT)GATCCATGTTTGTGTAGATCAAAATCTGGAA NGG 10 8 17 8 24 0 100 5 10
    240607004656_1jlo CTGTATCTACCCAGGCTATGAGCCAGGATCTG(+ATATAT)GTACCACCCTCCTTCTGTACCAAGAACACTG NGG 10 8 17 8 24 0 100 5 10
    240607004655_wsq6 TACCACAGTGTTCTTGGTACAGAAGGAGGGTG(+ATATAT)GTACCAGATCCTGGCTCATAGCCTGGGTAGA NGG 10 8 17 8 24 0 100 5 10
    240607004653_54nc CCCAGCAGGGGCAGGAGAGGACTGGTCTCATG(+ATATAT)GATCCCCTCTCCCCTGTTCTGCGGGCACCTC NGG 10 8 17 8 24 0 100 5 10
    240607004651_0coo CAGCGGAGGTGCCCGCAGAACAGGGGAGAGGG(+ATATAT)GATCCATGAGACCAGTCCTCTCCTGCCCCTG NGG 10 8 17 8 24 0 100 5 10
    240607004650_cxmd TTCTGTGTGTAGGGGTCTGTCCATGATGTTGG(+ATATAT)GTACCACCTGAATTAATCCCGGATTTTGTTA NGG 10 8 17 8 24 0 100 5 10
    240607004648_v3zw ACACATAACAAAATCCGGGATTAATTCAGGTG(+ATATAT)GTACCCAACATCATGGACAGACCCCTACACA NGG 10 8 17 8 24 0 100 5 10
    240607004647_if0s ACACACAGCATGTAATTAACAACAGATCTATG(+ATATAT)GATCCAGCTCTTAATATTGATCCTTTTGGTG NGG 10 8 17 8 24 0 100 5 10
    240607004646_ab5r AACACCACCAAAAGGATCAATATTAAGAGCTG(+ATATAT)GATCCATAGATCTGTTGTTAATTACATGCTG NGG 10 8 17 8 24 0 100 5 10
    240607004645_5wpx ATGTAGAGTCTCTTAGAGGACAGCCATACTAG(+ATATAT)GATCCTATCTGCAAGCACAACATGGCATCAG NGG 10 8 17 8 24 0 100 5 10