Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607004811_qajq CAGGCAAAGGTGCTTGCTGCCAATCCTGACTG(+ATATAT)GATCCACTGTAAAGAGAACCATTTCTCGACA NGG 10 8 17 8 24 0 100 5 10
    240607004809_2bga GCAAACAGCAAGAGGCTTTATTGAGAACACGG(+ATATAT)GTACCCGGGCGACGCAGTCTATCGGAGGACT NGG 10 8 17 8 24 0 100 5 10
    240607004807_rvrs GCGCCAGTCCTCCGATAGACTGCGTCGCCCGG(+ATATAT)GTACCCGTGTTCTCAATAAAGCCTCTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240607004805_x978 TAAGAGAAAACTGACAAACTACTTAATGAAGG(+ATATAT)GTACCAAGAAAGGACAATCGGATTCAGTTCG NGG 10 8 17 8 24 0 100 5 10
    240607004803_jefo TCTAGCGAACTGAATCCGATTGTCCTTTCTTG(+ATATAT)GTACCCTTCATTAAGTAGTTTGTCAGTTTTC NGG 10 8 17 8 24 0 100 5 10
    240607004802_jrdc GAGCTGCCAGCTCCTAGGGACAGATGCTAGAG(+ATATAT)GTACCCACAGCTCCCCTGAAGACCTCGTTTC NGG 10 8 17 8 24 0 100 5 10
    240607004800_it6e ACTGAGAAACGAGGTCTTCAGGGGAGCTGTGG(+ATATAT)GTACCTCTAGCATCTGTCCCTAGGAGCTGGC NGG 10 8 17 8 24 0 100 5 10
    240607004758_ku0y TTTTCTTTGTTGAAACTCTATGAATACCACAG(+ATATAT)GTACCTACGTGGCCCATGGCTGGTAACTATT NGG 10 8 17 8 24 0 100 5 10
    240607004757_jqyz CCCAAAATAGTTACCAGCCATGGGCCACGTAG(+ATATAT)GTACCTGTGGTATTCATAGAGTTTCAACAAA NGG 10 8 17 8 24 0 100 5 10
    240607004756_3iq5 CCAGAGAGTGACCCGGTTGCCAACCATAGAGG(+ATATAT)GTACCTATGATTCTTCATTTCTCAGGTTTGC NGG 10 8 17 8 24 0 100 5 10