Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607003450_gt2v GGAATGTCGCTTCATTGTATATTTTAGGCATG(+ATATAT)GTACCAACAAATATGTATCAAAAAAACATTT NGG 10 8 17 8 24 0 100 5 10
    240607003449_yhnk AGGAAAACAGAACACGTAAAAGTGTATTACAG(+ATATAT)GATCCAGAAAGGAAGAAAACAACTTTGGGTC NGG 10 8 17 8 24 0 100 5 10
    240607003448_6km1 AGCAAGACCCAAAGTTGTTTTCTTCCTTTCTG(+ATATAT)GATCCTGTAATACACTTTTACGTGTTCTGTT NGG 10 8 17 8 24 0 100 5 10
    240607003446_fmpk CCAGAGTCTAAGGGGATTCGATCCTGTGACAG(+ATATAT)GTACCTGGAGGTACACTTCACAGAAAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240607003445_uvxz GATGCTTCTCTTTCTGTGAAGTGTACCTCCAG(+ATATAT)GTACCTGTCACAGGATCGAATCCCCTTAGAC NGG 10 8 17 8 24 0 100 5 10
    240607003444_xjbp CTAACATACTTCCTTTGAGTGACATGAAATTG(+ATATAT)GTACCGTTGCTTGTGATGGCGGAGACGTAGT NGG 10 8 17 8 24 0 100 5 10
    240607003442_v38e ACCTCACTACGTCTCCGCCATCACAAGCAACG(+ATATAT)GTACCAATTTCATGTCACTCAAAGGAAGTAT NGG 10 8 17 8 24 0 100 5 10
    240607003441_sbnr CGCCGGCGGCGGCGACGTGTTCATAGCGCTGG(+ATATAT)GTACCACGCACCGGGAGTTCGCCCAAAGGAT NGG 10 8 17 8 24 0 100 5 10
    240607003439_4vfe GAAGCATCCTTTGGGCGAACTCCCGGTGCGTG(+ATATAT)GTACCCAGCGCTATGAACACGTCGCCGCCGC NGG 10 8 17 8 24 0 100 5 10
    240607003437_qipl AGGATATAAAGGGAACTAGCTTTTAAATAGGG(+ATATAT)GTACCACTGGTGGCCAGGGACTCTTGGTCTT NGG 10 8 17 8 24 0 100 5 10