Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607003347_drwa TTCTGGTCACAGAACACAGGCATTGGTGAATG(+ATATAT)GATCCGCTTCGCGTGGCACATCTGTAAGGTG NGG 10 8 17 8 24 0 100 5 10
    240607003346_u1ka AGGGTACTTGTGGAGGCCAGAAGAGGGTATTG(+ATATAT)GATCCTCTGGGGCTGGAGTTATGGGCAGCTG NGG 10 8 17 8 24 0 100 5 10
    240607003344_pa3p GCTTCCAGCTGCCCATAACTCCAGCCCCAGAG(+ATATAT)GATCCAATACCCTCTTCTGGCCTCCACAAGT NGG 10 8 17 8 24 0 100 5 10
    240607003343_9y5z TTTGGAGCCTTAGTATGTTTATTTTGTGGGAG(+ATATAT)GATCCGCACGCACACAGCTCACTGGAGGGAG NGG 10 8 17 8 24 0 100 5 10
    240607003341_vmcr GTCATCTCCCTCCAGTGAGCTGTGTGCGTGCG(+ATATAT)GATCCTCCCACAAAATAAACATACTAAGGCT NGG 10 8 17 8 24 0 100 5 10
    240607003339_buu7 GGTTTGGTTCTTTCTTAGTACTCACAGTGGGG(+ATATAT)GATCCTGTGGCTCTGATGCTGTCTTCTGACC NGG 10 8 17 8 24 0 100 5 10
    240607003336_zxww ATGCAGGTCAGAAGACAGCATCAGAGCCACAG(+ATATAT)GATCCCCCACTGTGAGTACTAAGAAAGAACC NGG 10 8 17 8 24 0 100 5 10
    240607003334_ndak AGCAATTTAGAGCACTGACTGCTCTTCCTCAG(+ATATAT)GATCCAGGTTTGACTCCCAGTAACCATATGA NGG 10 8 17 8 24 0 100 5 10
    240607003332_mzrf AAGTTTCATATGGTTACTGGGAGTCAAACCTG(+ATATAT)GATCCTGAGGAAGAGCAGTCAGTGCTCTAAA NGG 10 8 17 8 24 0 100 5 10
    240607003331_b0le TGCCTGTCACTTACTTACTGCTCGGTTGATAG(+ATATAT)GTACCACACAGCATCTCCATTTCTTCCAGGC NGG 10 8 17 8 24 0 100 5 10