Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607003435_qkyb CTGAGAAGACCAAGAGTCCCTGGCCACCAGTG(+ATATAT)GTACCCCTATTTAAAAGCTAGTTCCCTTTAT NGG 10 8 17 8 24 0 100 5 10
    240607003433_x0tp CCACAGCACAGAGATACCCACCAGTGTTCCAG(+ATATAT)GTACCCAGCAAGCTAACTAGCATAGACCTGG NGG 10 8 17 8 24 0 100 5 10
    240607003431_cy1v TTATCCCAGGTCTATGCTAGTTAGCTTGCTGG(+ATATAT)GTACCTGGAACACTGGTGGGTATCTCTGTGC NGG 10 8 17 8 24 0 100 5 10
    240607003430_82kz TGATATATTTCCCTACCTGCATAGCATGAATG(+ATATAT)GTACCCAGCTGCTATCTTGTGTTTCAACAAT NGG 10 8 17 8 24 0 100 5 10
    240607003428_f0ov AAGCCATTGTTGAAACACAAGATAGCAGCTGG(+ATATAT)GTACCATTCATGCTATGCAGGTAGGGAAATA NGG 10 8 17 8 24 0 100 5 10
    240607003426_d587 GACCCACATGCATATATTAGCCAGAGCTTGGG(+ATATAT)GTACCCTGTGTAAGAGTAGTAGGATGGATTG NGG 10 8 17 8 24 0 100 5 10
    240607003425_4u05 TCTTACAATCCATCCTACTACTCTTACACAGG(+ATATAT)GTACCCCAAGCTCTGGCTAATATATGCATGT NGG 10 8 17 8 24 0 100 5 10
    240607003424_beib GAATACCACATGCGTTTTTGTCTAATGCTTAG(+ATATAT)GTACCAAAATGTTAGCATCAGTATTCACTAT NGG 10 8 17 8 24 0 100 5 10
    240607003422_pt8w GCTCTATAGTGAATACTGATGCTAACATTTTG(+ATATAT)GTACCTAAGCATTAGACAAAAACGCATGTGG NGG 10 8 17 8 24 0 100 5 10
    240607003421_moot TGGTATCTGGAAGTCTGTGCACAAGATCTGAG(+ATATAT)GTACCATTGGGTGGGCTGAGATCTTTAAGTG NGG 10 8 17 8 24 0 100 5 10