Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607002653_jsma GACCTGCATCCCCTAAGTGAGGACCCTACAGG(+ATATAT)GTACCTTGTGCTCTGCCCCCAGACCCATAGC NGG 10 8 17 8 24 0 100 5 10
    240607002652_q60x CTAAGGCTATGGGTCTGGGGGCAGAGCACAAG(+ATATAT)GTACCCTGTAGGGTCCTCACTTAGGGGATGC NGG 10 8 17 8 24 0 100 5 10
    240607002650_mk65 TCCTTGGGGGTCTGGCAGGTGGCCACACCACG(+ATATAT)GATCCTTGAGCTGTCTCGAGGGTCAGGCCAG NGG 10 8 17 8 24 0 100 5 10
    240607002649_g8mq TGCCACTGGCCTGACCCTCGAGACAGCTCAAG(+ATATAT)GATCCGTGGTGTGGCCACCTGCCAGACCCCC NGG 10 8 17 8 24 0 100 5 10
    240607002647_geq6 GCTGGCTGCTCTCCTCCCTGCAGTCCACCCTG(+ATATAT)GATCCCTCGCCTCTAGGGAACAGAAGTTTAC NGG 10 8 17 8 24 0 100 5 10
    240607002645_f6x2 CCAGCGTAAACTTCTGTTCCCTAGAGGCGAGG(+ATATAT)GATCCAGGGTGGACTGCAGGGAGGAGAGCAG NGG 10 8 17 8 24 0 100 5 10
    240607002642_2l8j CTCTAAGTTGCTCTGACTAGCCTTGAGCCCAG(+ATATAT)GATCCTCATGAAGGCTGAGATTATAAATATA NGG 10 8 17 8 24 0 100 5 10
    240607002641_3huh GAATTTATATTTATAATCTCAGCCTTCATGAG(+ATATAT)GATCCTGGGCTCAAGGCTAGTCAGAGCAACT NGG 10 8 17 8 24 0 100 5 10
    240607002640_14id CACCTCTAACCCCAGCACTGAGGGAATAGGTG(+ATATAT)GATCCAAGGGGTTCTCTGGACAAGTGGTCTA NGG 10 8 17 8 24 0 100 5 10
    240607002638_y4bp TGGGCTAGACCACTTGTCCAGAGAACCCCTTG(+ATATAT)GATCCACCTATTCCCTCAGTGCTGGGGTTAG NGG 10 8 17 8 24 0 100 5 10