Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607002547_dl7y GTGACGCTCAGATCTGAGAAGAAACATGATGG(+ATATAT)GATCCATATCAGGTTGTGAATATTTTTTTGA NGG 10 8 17 8 24 0 100 5 10
    240607002546_8yrb TGTTCTCAAAAAAATATTCACAACCTGATATG(+ATATAT)GATCCCATCATGTTTCTTCTCAGATCTGAGC NGG 10 8 17 8 24 0 100 5 10
    240607002544_mzz0 TAAAGTACTATGACACTAGAACCTTTGCAGAG(+ATATAT)GATCCAACTACAATGTAAGGTTGATCTTCCA NGG 10 8 17 8 24 0 100 5 10
    240607002543_de1s GATACTGGAAGATCAACCTTACATTGTAGTTG(+ATATAT)GATCCTCTGCAAAGGTTCTAGTGTCATAGTA NGG 10 8 17 8 24 0 100 5 10
    240607002541_xenj AGAGCGGTCACTGGACTAGGATGCATCCCTTG(+ATATAT)GATCCTGGGATAGGCTCTGATCGGACCATAA NGG 10 8 17 8 24 0 100 5 10
    240607002538_k312 AACACTTATGGTCCGATCAGAGCCTATCCCAG(+ATATAT)GATCCAAGGGATGCATCCTAGTCCAGTGACC NGG 10 8 17 8 24 0 100 5 10
    240607002537_l1mi TTTTAAGACTAGCCACAGGCTATGAATATATG(+ATATAT)GTACCCCTTTGAAGGTAAAAATTGAAGCAAT NGG 10 8 17 8 24 0 100 5 10
    240607002535_9a3f AAAACATTGCTTCAATTTTTACCTTCAAAGGG(+ATATAT)GTACCATATATTCATAGCCTGTGGCTAGTCT NGG 10 8 17 8 24 0 100 5 10
    240607002534_gidg GATTAAATTCGGGTCATCAGGATTGGCAAGAG(+ATATAT)GTACCTTTACCTGCTGAGTCATTGCATTGGC NGG 10 8 17 8 24 0 100 5 10
    240607002532_62v1 ATTAGGCCAATGCAATGACTCAGCAGGTAAAG(+ATATAT)GTACCTCTTGCCAATCCTGATGACCCGAATT NGG 10 8 17 8 24 0 100 5 10