Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607002524_8gcw CTGAGAAAGAGAAAGGTGTCTGAGTAATTCTG(+ATATAT)GATCCAGTTTTTTCCCCTAGAAAGACTGAGA NGG 10 8 17 8 24 0 100 5 10
    240607002523_vkw4 GTAAGGAAGCAGAGCCCAGGAGCTCAAAGCTG(+ATATAT)GTACCCAGAAGAGCCAGCAGGGCCAGGCTAA NGG 10 8 17 8 24 0 100 5 10
    240607002521_x9o5 AGTCCTTAGCCTGGCCCTGCTGGCTCTTCTGG(+ATATAT)GTACCAGCTTTGAGCTCCTGGGCTCTGCTTC NGG 10 8 17 8 24 0 100 5 10
    240607002520_9xas GGTGACCTGCCATCTGTGACAAGCTTTCTCGG(+ATATAT)GTACCTCTCCCCACCTCATTCCTTTAGGCTA NGG 10 8 17 8 24 0 100 5 10
    240607002518_vvwa CTCCATAGCCTAAAGGAATGAGGTGGGGAGAG(+ATATAT)GTACCCGAGAAAGCTTGTCACAGATGGCAGG NGG 10 8 17 8 24 0 100 5 10
    240607002517_gdnt TGTAATTTTTGTATATAATGGGCCAAATTAAG(+ATATAT)GATCCTGCTCTCTTTCTTTTGGAGGTAGTTT NGG 10 8 17 8 24 0 100 5 10
    240607002516_35oj ACCTCAAACTACCTCCAAAAGAAAGAGAGCAG(+ATATAT)GATCCTTAATTTGGCCCATTATATACAAAAA NGG 10 8 17 8 24 0 100 5 10
    240607002515_0k1c GCATGTATTCAGTTTTAGAATCACTTTTACTG(+ATATAT)GATCCTGTAGTAATGTCTAGAAGTATCATAT NGG 10 8 17 8 24 0 100 5 10
    240607002514_bp46 AATAGATATGATACTTCTAGACATTACTACAG(+ATATAT)GATCCAGTAAAAGTGATTCTAAAACTGAATA NGG 10 8 17 8 24 0 100 5 10
    240607002512_4d8j TTACTTGAAAATTGGACATAGTACTACCAGAG(+ATATAT)GATCCCACAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10