Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607002345_xl1i ATAGAAGGCAGAGGAATTATTTGGGAAGAGGG(+ATATAT)GATCCTCTCCAGGCTCTTAGCCAAACAAGGG NGG 10 8 17 8 24 0 100 5 10
    240607002344_nbz2 TGTGAGAATGGGGATTGATGAGGCCCAGCTTG(+ATATAT)GTACCTGGAAAATGTGTATGGTAAGGAAAAT NGG 10 8 17 8 24 0 100 5 10
    240607002342_2vj8 TGCACATTTTCCTTACCATACACATTTTCCAG(+ATATAT)GTACCAAGCTGGGCCTCATCAATCCCCATTC NGG 10 8 17 8 24 0 100 5 10
    240607002340_e3w8 AGAGACTGCCTGGAGATGGACAGAGAAGCTGG(+ATATAT)GATCCTTGGTGACCAGCCCCTCTGAAACACT NGG 10 8 17 8 24 0 100 5 10
    240607002339_ul4j CCTAGAGTGTTTCAGAGGGGCTGGTCACCAAG(+ATATAT)GATCCCAGCTTCTCTGTCCATCTCCAGGCAG NGG 10 8 17 8 24 0 100 5 10
    240607002336_8mxp AACACTCCCTCAGTCCCAGAAGTTTCCCCACG(+ATATAT)GATCCCCATTTGCATATAACCTCTTCCCATC NGG 10 8 17 8 24 0 100 5 10
    240607002334_8xxn GGTAGGATGGGAAGAGGTTATATGCAAATGGG(+ATATAT)GATCCGTGGGGAAACTTCTGGGACTGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240607002333_07gf AGGAAATGGGAGGGAGATGAGAAAACAGGATG(+ATATAT)GTACCAATCACCTGGGTAATCAACTGGTTGA NGG 10 8 17 8 24 0 100 5 10
    240607002332_zfm2 CCTCTTCAACCAGTTGATTACCCAGGTGATTG(+ATATAT)GTACCATCCTGTTTTCTCATCTCCCTCCCAT NGG 10 8 17 8 24 0 100 5 10
    240607002330_w27n ACTACATGGTGGCTTGCAATCATCTGTAATGG(+ATATAT)GATCCAATGCCTTCTTTGGGTGAATCTGAAG NGG 10 8 17 8 24 0 100 5 10