Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607002315_mscf TCTATGCCAATATTTGTTTAAAATACATCTTG(+ATATAT)GATCCAACACACAAGTAATTGGACCAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240607002314_tb53 AATTGTAACATAGGTAGTATGCCTTCACACTG(+ATATAT)GATCCCGGGGGCATGGGTTTTGGAGATGTTA NGG 10 8 17 8 24 0 100 5 10
    240607002311_w7hp CAAAATAACATCTCCAAAACCCATGCCCCCGG(+ATATAT)GATCCAGTGTGAAGGCATACTACCTATGTTA NGG 10 8 17 8 24 0 100 5 10
    240607002309_vx6v CACCACCTTGCTACGTGCATGTACCAGGCAAG(+ATATAT)GTACCAAAGATAACAAATGAAAAACAAACCC NGG 10 8 17 8 24 0 100 5 10
    240607002308_cxae ATCATGGGTTTGTTTTTCATTTGTTATCTTTG(+ATATAT)GTACCTTGCCTGGTACATGCACGTAGCAAGG NGG 10 8 17 8 24 0 100 5 10
    240607002307_u8r6 AATCAGCTTGCTTTGTTGGCCTAGATATTTTG(+ATATAT)GATCCTTTATTGAAATGAGGCAGTGAGATAG NGG 10 8 17 8 24 0 100 5 10
    240607002306_amn5 GGAAACTATCTCACTGCCTCATTTCAATAAAG(+ATATAT)GATCCAAAATATCTAGGCCAACAAAGCAAGC NGG 10 8 17 8 24 0 100 5 10
    240607002305_5gbn AGTGGAAATAATTTGCTGTCGTTTCAGACTTG(+ATATAT)GATCCTACAAGCCTGTCCGTTCTGTAAGTGG NGG 10 8 17 8 24 0 100 5 10
    240607002303_i2rf CAGTACCACTTACAGAACGGACAGGCTTGTAG(+ATATAT)GATCCAAGTCTGAAACGACAGCAAATTATTT NGG 10 8 17 8 24 0 100 5 10
    240607002302_jgvk TGACAAGGGAGTCGGAAGCTAAGGAGGTGACG(+ATATAT)GATCCTAATGCTTGTCAAATAAACCGATCGC NGG 10 8 17 8 24 0 100 5 10