Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607002444_h1gp GGTCCAAACAACACATCATCATGATGTCCCAG(+ATATAT)GATCCACTATGTCCTCATTGCTTCCCAGAAT NGG 10 8 17 8 24 0 100 5 10
    240607002443_dg6r GAAGGATTCTGGGAAGCAATGAGGACATAGTG(+ATATAT)GATCCTGGGACATCATGATGATGTGTTGTTT NGG 10 8 17 8 24 0 100 5 10
    240607002442_toc9 GCTCAGGAAAGGGTGATAATGGATAGGATGCG(+ATATAT)GATCCCTAAGTAGATGGGATAAATGTATTAC NGG 10 8 17 8 24 0 100 5 10
    240607002440_cd5p CGTGTGTAATACATTTATCCCATCTACTTAGG(+ATATAT)GATCCGCATCCTATCCATTATCACCCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240607002437_n2qw ACCAGCCCAAGACATTTTTACTTTTCTCCTGG(+ATATAT)GATCCAAAGAAATTCTGTGCTTCATGAGAAC NGG 10 8 17 8 24 0 100 5 10
    240607002436_nrbc TCTTAGTTCTCATGAAGCACAGAATTTCTTTG(+ATATAT)GATCCCAGGAGAAAAGTAAAAATGTCTTGGG NGG 10 8 17 8 24 0 100 5 10
    240607002435_2swv ACACACATTTATATGCTATATCTGTATTAAGG(+ATATAT)GATCCTCCAGGGTTGCCCTATTCTTCACCTC NGG 10 8 17 8 24 0 100 5 10
    240607002433_e8lb CACAGGAGGTGAAGAATAGGGCAACCCTGGAG(+ATATAT)GATCCCTTAATACAGATATAGCATATAAATG NGG 10 8 17 8 24 0 100 5 10
    240607002432_p6dd GGAGTATGCCGAAAGGATCGGAGTCTAAACTG(+ATATAT)GTACCCTAGCATTTCAAAGGTCCTCAACAGA NGG 10 8 17 8 24 0 100 5 10
    240607002430_jtfj AAGTATCTGTTGAGGACCTTTGAAATGCTAGG(+ATATAT)GTACCAGTTTAGACTCCGATCCTTTCGGCAT NGG 10 8 17 8 24 0 100 5 10