Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607002301_bpqf TTTTGGCGATCGGTTTATTTGACAAGCATTAG(+ATATAT)GATCCGTCACCTCCTTAGCTTCCGACTCCCT NGG 10 8 17 8 24 0 100 5 10
    240607002300_2uh3 TATCCGTGCTGGAATGGAATGTTAACTGACAG(+ATATAT)GATCCTGTGTGTGTGTGTGTGTGTGTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240607002258_gl58 ACACACACACACACACACACACACACACACAG(+ATATAT)GATCCTGTCAGTTAACATTCCATTCCAGCAC NGG 10 8 17 8 24 0 100 5 10
    240607002257_oveu TATGCGATGGTCTAATAATTAAACGATACTGG(+ATATAT)GTACCCAATGAGTTTTCAGAGACTGCTGTGT NGG 10 8 17 8 24 0 100 5 10
    240607002255_qqw7 CTTGTACACAGCAGTCTCTGAAAACTCATTGG(+ATATAT)GTACCCAGTATCGTTTAATTATTAGACCATC NGG 10 8 17 8 24 0 100 5 10
    240607002253_tbdv TTGGCACAAACTGGAGGATTTTACTGTCCATG(+ATATAT)GTACCTTTGCTGGATCCTTTCCCAAAGACAG NGG 10 8 17 8 24 0 100 5 10
    240607002252_ttdt TAACACTGTCTTTGGGAAAGGATCCAGCAAAG(+ATATAT)GTACCATGGACAGTAAAATCCTCCAGTTTGT NGG 10 8 17 8 24 0 100 5 10
    240607002251_lhse TATGTAGAATATACTGGTCTCCAACTCACAAG(+ATATAT)GATCCACCTGCCTCTGCCTCCTGAGGGGTGA NGG 10 8 17 8 24 0 100 5 10
    240607002249_rq72 CAGTCTCACCCCTCAGGAGGCAGAGGCAGGTG(+ATATAT)GATCCTTGTGAGTTGGAGACCAGTATATTCT NGG 10 8 17 8 24 0 100 5 10
    240607002248_0o5w TGAGCTGAGCGTCTCTATCCTTCCCTGTTCAG(+ATATAT)GATCCTTCCTGCAGGTGCTCATGAGATAGTA NGG 10 8 17 8 24 0 100 5 10