Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607002221_htyp TTAGTTCATATGTTAGCAGGCAGCGGCAGATG(+ATATAT)GATCCACTGCAAACACTTCACAGATACACCA NGG 10 8 17 8 24 0 100 5 10
    240607002219_lk52 GTTCCAGGAGCCTCCTCATGTTTGCCTGCTGG(+ATATAT)GATCCCTGCACAAGGTGTTTCTTTACAATAG NGG 10 8 17 8 24 0 100 5 10
    240607002217_od7q CTGGTCTATTGTAAAGAAACACCTTGTGCAGG(+ATATAT)GATCCCAGCAGGCAAACATGAGGAGGCTCCT NGG 10 8 17 8 24 0 100 5 10
    240607002215_mnl6 AATACTACCATAAATAACTAGCACCCACCAAG(+ATATAT)GATCCCAAGTTCCTGTCTTTTCCAAATGGCT NGG 10 8 17 8 24 0 100 5 10
    240607002214_c7b3 ACCCTAGCCATTTGGAAAAGACAGGAACTTGG(+ATATAT)GATCCTTGGTGGGTGCTAGTTATTTATGGTA NGG 10 8 17 8 24 0 100 5 10
    240607002212_cpup GTCAGGAGAACACTTGAAAGGCTTTCTTTGTG(+ATATAT)GTACCTACGGGGTGAAATCACAGCCTGTCAA NGG 10 8 17 8 24 0 100 5 10
    240607002210_tg6x CTCTCTTGACAGGCTGTGATTTCACCCCGTAG(+ATATAT)GTACCACAAAGAAAGCCTTTCAAGTGTTCTC NGG 10 8 17 8 24 0 100 5 10
    240607002209_t9zi ATTTGAAATGGTTGATAAAATCCTCAAGCTAG(+ATATAT)GATCCCCCAGAAGGTGTTTGATTGCTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240607002207_fldp AAGGGAACAAAGCAATCAAACACCTTCTGGGG(+ATATAT)GATCCTAGCTTGAGGATTTTATCAACCATTT NGG 10 8 17 8 24 0 100 5 10
    240607002206_4w9q ATCATCTTACCATTCTGATCCTTCATGCTCTG(+ATATAT)GTACCCGAGCTCGGAGCGCCCCATCCGTCTA NGG 10 8 17 8 24 0 100 5 10